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Microscope with epi-fluorescent is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances. The “fluorescence microscope” refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope, or a more complicated design such as a confocal microscope or lightsheet microscope, which uses optical sectioning to get better resolution of the fluorescent image.

Fluorescence microscopy is not just generating one, two, three or more single or multiple color images with the dark background! This is an enormously powerful technique to investigate biological questions, dig more and more on the details. Most of the researchers push the microscopes to the maximum sensitivity level and resolution, when it comes to fluorescence imaging techniques.  It is important to consider the objective lens numerical aperture (NA), proper filter, filter coating quality, light source’s power, optics to collimate the light, camera sensitivity level, and manual setup of the microscope as well as the fluorochrome staining techniques to achieve a high-quality fluorescence microscope.

Applications of fluorescence microscope are (but not limited to): biology research, cancer research, neuroscience, ADME or Toxicology, fluorescence in situ hybridization (FISH), tissue microarrays, biomedical contract research, any fluorescent-labelled particles or beads involved project

4 Things I wish I know before purchasing  a fluorescence microscope:

1. What fluorophores or filters do I need?

  • Emission color is normally one color/bandwidth above the excitation channel. For instances, the excitation for GFP is blue but emission is green. Similarly, we use green excitation but red emission filter for RFP/mCheery/TexasRed filters.
  • We have filters from Visible to far red and infrared (300nm-1800nm), over 150 sets.

2.  Do I need a multi-band filter? A dual, triple and even 5-band multi-wavelength filters are available to collect multiple channels in one shot.

3. What light source: LED or HBO? How about Xenon?

  • HBO is a traditional light source with a wide spectrum of wavelength for the visible range at low cost and around 200-3000 hours lifetime.
  • LED source is available from UV to IR, at the selected wavelength, with little bit extra cost from 10,000 hours lifetime up to 100,000 hours ( you will be retired from the job).

4. What type of microscope am I better to choose: stereoscope, inverted or upright? please read each category separately to find the answers.

5. More questions? Simply contact us by phone, email or live chat.

Please select the products based on your requirements from the left side list or below table:

Inverted FluorescenceUpright FluorescenceStereo FluorescenceDigital Fluorescence
BUM500DIC Upright Biological Microscope with DIC and Phase Contrast Imaging-10756
Heating StagesFluorescence AccessoriesObjective LensesOEM

A highly recommended accessory for all fluorescence microscope is fluorescent alignment objective lens:

Epi-fluorescent Illumination Alignment Tool / Objective Lens

Video Tutorial:

Note: This is is an advanced video tutorial. An introduction video is posted in Inverted Fluorescence and Upright Fluorescence.

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