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Inverted Biological Microscopes

Universal Manual XY Stage for inverted biological microscopes

If you are dealing with cell culture, handling liquids in your device, an inverted biological microscope is an essential tool to have. One likes to make a video of living cells to know how they grow, divide or die and its rate, you need an inverted biological microscope, a digital camera, and software for the time-lapse mode.

What to consider in selecting an inverted fluorescence microscope?

 Objective lenses-magnification: normally the inverted microscopes come with 10x, 20x and 40x obj lenses, some models include 4x. A 4x obj lens allows seeing an entire field of a well in a 96-well plate. You can get obj lenses from 1.25x till 150x and even more.

Objective lenses-Working distance: Working distance (WD) of an objective lens is defined as a distance from the edge of the lens until the specimen. If you use a 100x/NA 1.25/WD: 0.12mm to see tissue culture cells inside a well of a 96-well plate, you will see nothing! Why? Because the thickness of well plate plastic is around 0.5mm which is longer than lens WD=0.12mm.

 Phase Contrast: generally speaking 10x, 20x and 40x obj lenses with Phase Contrast are available. Depending on the model you can expect a 4x Ph as well.

 Halogen or LED?

 Upgrade: Think of long future use of the microscope. Can you upgrade it to have more features? Having fluorescence imaging capability, switching to LED, more spots for extra obj lenses, might be having polarized, darkfield, and probably DIC Nomarski and so are examples of the questions we ask our clients before suggesting a model.

BIM200 Inverted Biological Microscope

Bioimager’s Inverted Biological Microscopes are offered in two main groups:

1. Portable Microscopes: Compact Design, fits inside hood and incubator, such as IncuScope, BMI200, UniVis-100, UNIVIS-500.

2. Lab Microscopes such as BIM500

we have recently developed a polarization kit for inverted biological microscope such as BIM500P which can be customized for other models.

Mutant Jeans

Video Tutorial: How to setup an inverted Biological Microscope

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