Custom Design of A Miniature Incubator /Chamber for Automated Upright Microscope BMU450A
Here we describe how to make a custom miniature incubator or chamber for an upright automated/manual biological/industrial microscope. The chamber allows control of temperature in the base, lid and objective lens, cooling and/or heating, humidity and CO2/O2 and most importantly scanning a big area of a sample such as one or two 35mm petri-dishes, well plate, chamber slides and so on, in an automated mode while the chamber is fully closed. Here we discuss some challenges during the development in terms of how to fit this chamber to the microscope and meet all requirements of having a closed chamber for a motorized stage.SKU: n/a
Why do you need a live cell imaging chamber?
A live cell imaging chamber is in fact a miniaturized version of an incubator which can be mounted on top of the stage of a microscope while capturing the events occur for your cells. If you plan to do long-term live cell imaging, you would definitely need such a chamber which provides the humidity and gas for your cells and also keeps the temperature in an optimum value. Thus, three parameters of temperature, gas (CO2, O2 and N2) and humidity need to be controlled as in most of the cases very precisely.
Live cell imaging chambers are specialized devices designed to maintain live cells in a controlled and stable environment while they are being imaged under a microscope. These chambers allow researchers to monitor and analyze dynamic cellular processes, such as cell division, migration, and differentiation, over time.
There are several types of live cell imaging chambers available, including:
- Open dish chambers: These chambers consist of a simple dish or plate that is placed on the microscope stage. The dish can be filled with a liquid medium to maintain cell viability and provide nutrients. This type of chamber is simple and inexpensive, but it can result in evaporation and fluctuations in temperature and CO2 levels.
- Closed perfusion chambers: These chambers are designed to maintain a continuous flow of fresh medium over the cells, allowing for stable and uniform conditions. They typically have input and output ports to control the flow of medium and the levels of gases, such as oxygen and CO2. Closed perfusion chambers are more complex and expensive than open dish chambers, but they provide more precise and stable conditions.
- Microfluidic chambers: These chambers use microfluidic technology to create complex fluidic environments, such as gradients, shear stress, or confined spaces, that mimic physiological conditions. Microfluidic chambers can be used to study a wide range of cellular processes, such as chemotaxis, cell-cell interactions, and drug responses. However, they require specialized equipment and expertise to operate.
Live cell imaging chambers can be used with various imaging techniques, including fluorescence microscopy, confocal microscopy, and time-lapse microscopy. They are widely used in cell biology, developmental biology, cancer research, and drug discovery, among other fields. The choice of the chamber depends on the specific experimental requirements and budget constraints.
Tips and Hints: What do you need to have to be able to get clear, stable and long-term images of your cells?
For most of the cell imaging, you will need at least these items:
- A physical chamber, in which you can culture your cells. The chamber should allow easily installation and removal of the cell culture in a container such as well-plate, petri-dish, tissue culture flasks, micro-slide etc.
- A gas supplier, which can be a digital or analogue gas mixer which provides the gas for your cells. The types include a mixed of CO2 and air, and mixed of CO2, O2, and N2.
- A temperature controller, which allows to control temperature inside the chamber, outside the chamber and also surrounding the objective lenses to avoid drift focus (otherwise you will see blurry images due to ambient temperature fluctuation over time).
- A gas humidifier, this is simply a flow of premixed gas passes through water in a closed system.
- A glass-bottom dish or plate
- A phenol-free medium
Why BIOIMAGER LCI Products?
BIOIMAGER provides very precisely manufactured chambers from the pioneer in this field, TokaiHit (a Japanese company).
Some Features of these products:
- Long-term stable environment
- Precise control temperature, gas mixing and humidity level
- Compact design
- Focus stability
- Clear vision, clear Image
- No big box needed
You do not know what to choose, simply use this Live Cell Imaging Selection Questionnaire, we will be more than happy to assist you.