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BiopenSPIM Light Sheet Microscope
- Customizable light-sheet microscope based on your application and budget
- The lowest price and most compact lightsheet microscope in the market
- Automated XY, Z and R (rotation)
- Customized chamber(s) according to the client’s sample(s)
- Big list of filters and lasers from UV to IR is available
- Objective lenses: 4x, 10x, 20x, 40x
- Camera: CMOS, CCD, sCMOS
- MicroManager Software
- Easy Upgradable from single to two colors, anytime
Selective Plane Illumination Microscopy (SPIM) Light Sheet
SPIM Principle
| The SPIM technology offers fast, optically-sectioning, minimally-invasive 3D acquisition of fluorescing specimen over time. It achieves that by focusing a thin laser light-sheet into the specimen, taking two-dimensional images of the illuminated slice with a perpendicularly positioned detector (CCD camera). Three-dimensional stacks are obtained by moving the specimen orthogonal to the light-sheet between consecutive images. By mounting the sample in a rigid medium, e.g. agarose, and hanging it into the sample chamber in front of the detection lens, it is possible to rotate the sample and collect 3d stacks from multiple angles (views). |  |
SPIM Applications
| SPIM promises to revolutionize several fields of biological research, in particular developmental and cell biology, by allowing imaging of large samples with high resolution over extended periods of time. SPIM has been used in a spectacular fashion to record the development of embryos of model organisms such as Drosophila and zebra fish with cellular resolution throughout the developing specimen (the so-called in toto imaging). The ability of SPIM to deliver high signal-to-noise 3D images of large specimen from different angles in an extended time-lapse is currently hard to achieve with any other microscopy technology. Since monitoring biological systems with high resolution over time is the goal of essentially all fields of biological inquiry, SPIM technology is imminently useful to biologists. |  |
Features
- Customizable light-sheet microscope based on your application and budget
- The lowest price and most compact lightsheet microscope in the market
- Automated XY, Z and R (rotation)
- Customized chamber(s) according to the client’s sample(s)
- Big list of filters and lasers from UV to IR is available
- Objective lenses: 4x, 10x, 20x, 40x
- Camera: CMOS, CCD, sCMOS
- MicroManager Software
- Easy Upgradable from single to two colors, anytime
BiopenSPIM Light-Sheet Microscope
Introduction
This is a complete ready to use industrial version of OpenSPIM (Open access Selective Plane Illumination Microscopy)-concept light-sheet microscope. The microscope includes one laser, two objective lenses, one motorized 4-axis stage (XY stage, autofocus, rotation), a chamber, a camera, c-mount adapter, USB hub and required cables, all installed inside a nice box. All of these parts can be customized, based on your imaging requirements, at a very reasonable price. This is the lowest price light sheet microscope package available in the market.
Specifications
1) Chamber
Customized per application and imaging requirements:
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2) Objective Lenses
We offer 4x, 10x, 20x and 40x. You need minimum two sets of these objective lenses, one for excitation and another for emission /detection.
3) Laser and Filter
3) Laser and Filter
4) Camera
CMOS, sCMOs and cooled CCD cameras are available.
5) 4-Dimensional XYZ-R Stage
5) 4-Dimensional XYZ-R Stage- Compact four dimensional (X, Y, Z, R) Stage.
- Draws power from a standard USB port with use of a externally powered USB 4-port Hub.
- Fully self-contained miniature (Size-8) stepper motors, with integrated control electronics.
- Linear (X, Y, Z) travel range of about 9 mm (~0.35”).
- Linear slides have friction locks for transport.
- Positional resolution of 1.5 microns (0.00006”).
- Rotational resolution of 200 step/rev (0.5 deg).
- Includes Sample-Arm with pulleys and belt to hold and rotate the Sample-Tube.
- Power efficient, holds position with no power.
- Built-in magnetic (Hall effect) home sensor on the linear (X, Y, and Z) motors.
- PC Windows based user interface included for easy “out of the box” motion control.
- Dynamic Linkable Library ( DLL) included for custom automated application.
Fruit fly
Histone-YFP from gastrulation to beginning of muscle contraction from ventral, lateral and dorsal view
Expression pattern of Csp-sGFP recorded with OpenSPIM
Drosophila blastoderm stage embryo – deconvolved
Drosophila blastoderm stage embryo – fused using content based fusion
Drosophila gastrulation timelapse 5 views every 6 minutes (HisYFP)
Drosophila gastrulation timelapse 5 views every 5 minutes (HisYFP)
His-YFP embryo imaged from gastrulation until muscle contractions in late embryogenesis – ventral view
A slice through a Drosophila embryo expressing HisYFP in all cells – unprocessed
Zebrafish
Multi-position z-stack of a living 48 hpf Tg(Bactin:H2A-EGFP) zebrafish, stitched using the Grid/Collection Plugin in Fiji. Maximum intensity projection, gamma 0.8.
Multi-position z-stack of a living 48 hpf Tg(Bactin:H2A-EGFP) zebrafish, stitched using the Grid/Collection Plugin in Fiji. Stack video.
Beating heart of a 48 hpf Tg(cmlc2:EGFP) zebrafish. Left: overlay of transmitted light and fluorescence signal. Right: fluorescence signal.
Volvox
Z-projection of a stack through a colony of Volvox carteri, imaging chlorophyll autofluorescence. The colony is approximately 330 microns wide.
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