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Phase Contrast is a contrast enhancing optical microscopy technique in which an unstained transparent sample is seen with high brightness by shifting phase difference of light.

The idea of Phase Contrast Imaging was brought by Frits Zernike in 1930’s, a Dutch physicist, who got the Nobel prize in 1953 for this invention.    

Phase contrast is mainly used by biologists and has some advantages over brighfield imaging. Using phase contrast microscopy, many cellular structures that cannot be seen by a brightfield imaging become visible, without staining.

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Video Tutorial: Adjusting Phase