IncuScope IS250 Incubator...
- Fits inside an incubator or a hood.
- Quadruple nosepiece, 10x, 25x and 40x Ph (and FL) with optional lenses of 2x-150x
- 2.3MP Color USB3.0 High-Sensitive Camera, C-mount adapter and calibration slide makes the imaging bundle so professional
- A trinocular head to use the scope in a classical way
- USB 3.0 connections for camera and LED Control
- LED Control via manual controller and software
- IncuView Software for LED Control (intensity, turn on/off) and time-lapse imaging and merging channels
5-Channel LED Fluorescence...
- LED fluorescence attachment
- Up to 5 channels of standard or custom wavelengths from UV to IR and NIR
- Standard 3-channel: UV, Blue and Green excitations, suitable for Dapi/Hoechst, GFP/Alexa Fluor 488, RFP/TRITC/mCherry/Texas Red.
- A 5W LED illumination
- Comes with touch-pad to select and turn up/down the LED intensity
- An adapter for Zeiss, Nikon, Leica, Olympus and custom microscopes of inverted, upright or stereo type.
- Optional LED control software for advanced live cell imaging
BIM800FLW Inverted Epi-Fluorescence...
Inverted Fluorescence Microscope
10x, 20xPh, 40x lenses incldued, with tens of optional LWD lenses
Positive & Negative Phase Contrast Lenses
GFP, RFP and Dapi Filters, mounted on a 5-position filter wheel
XY stage with 96-well plate markers
100W HBO/Mercuyry illuminationSKU: BIM800FLW
IncuScope IS500Ph Hoffman...
- Inverted Biological Microscope
- Brightfield and Phase-Contrats Imaging, with optional fluorescnce imaging
- Excellent Phase Contrats imaging; Hoffman Modulation Contratst,
- Puller-type phase contrast Condenser WD:55mm
- 10x Ph (incldued), and optional 20x Ph, 40x Ph, and brightfield 1.25x-500x objective lenses.
- 2.3 MP Built-in Camera
- Fits inside hood or incubator
- Great Time-Lapse Imaging Software with turn on/off lights (digital shutter)
Microscopes are used today by people from mid / high school students to the scientists and in several fields. It is even used as a daily routine work by biologists, physicians / hospital laboratory, geologists, dentists / dental technicians, veterinarians, paleontologists, entomologists, gemologists, hair transplant, scientific researchers, quality control personnel, quality assurance, assemblers, forensic document examiners, art restoration, textile specialists, wire and die manufacturers, environmental specialists, ophthalmic specialists, dermatologists, metrology, tool & die, circuit board rework, horticultural experts, solar industry, exterminators, fish and wildlife, US / Canada / EU / Asia customs, semiconductor manufacturers, foresters, pharmaceutical, electroplating and so on.
In this series of BIOIMAGER Microscope Tutorial, we like to discuss the all microscopy imaging modes that we hear / read a lot which includes Brightfield, Darkfield, Polarizing, Fluorescence, Phase Contrast, DIC Nomarski.
1. Brightfield (BF)
Brightfield (BF) is the simplest imaging technique of all the optical microscopy imaging mode. As the name states you can expect an image with bright background. A simple example in our daily life is like seeing birds or airplane in the sky. In BF imaging, a sample is normally illuminated via transmitted light coming from a white light and contrast in the sample is caused by the absorbance of some of transmitted light in dense areas of the sample.
2. Polarizing (Pol)
A type of optical microscopy techniques involving polarizing light. Simple techniques include illumination of the sample with polarized light. Directly transmitted light can, optionally, be blocked with a polarizer orientated at 90′ degrees to the illumination. more complex microscopy techniques which take advantage of polarized light include differential interference contrast (DIC Nomarski) microscopy and interference reflection microscopy.
Basic Polarized Light Microscopy Terminology:
Polar: A device which produces plane polarized light from natural light.
Plane-Polarized Light: Light with only on vibration direction present.
Polarizer: A polar placed in the light path before the specimen.
Analyzer: A polar place in the light path after the specimen. The analyzer is removable from the light path and may be rotatable. The analyzer is used to determine the optical effects produced by the specimen either in plane or polarized light.
Strain Free: A term used to signify that microscope objectives and condenser lenses are selected to have a minimum amount of internal stress in the glass. Strain-free optics offers little or no contribution to the optical path difference of the specimen.
Bertrand Lens: The Bertrand lens is located above the analyzer. the eyepiece and Bertrand lens act as a system to focus on the back image plane of the objective. The Bertrand lens’ main function is to view interference figures (conoscopic images) which appear in the back image plane of the objective when the specimen is viewed between crossed polars using highly convergent light from the condenser.
Birefringence: A quantitative expression of the separation of a light beam as it penetrates a doubly refracting object into two diverging beams.
Conoscopic Figure: A pattern consisting of isogyres and/or isochromatic curves formed in the back image plane of the objective also referred to as an interference figure.
Conoscopic Observation: Observation of the back focal plane of a light microscope objective with a Bertrand lens or phase telescope, using a cone of light from the condenser.
PleoChroism: A property exhibited by certain crystals of absorbing selectively various wavelengths of light and displaying different colors when looked at in the directions of their various crystal axes.
In optical microscopy, darkfield describes microscopy methods, in both light and electron microscopy, which exclude the unscattered beam from the image. As a result, the field around the specimen is generally dark. Darkfield microscopy is a very simple yet effective technique and well suited for uses involving live and unstained biological samples, such as a smear from a tissue culture or individual, water-borne, single-celled organisms. Darkfield optics are a low-cost alternative to phase contrast optics.
3. Phase Contrast (PH)
4.. Fluorescence Imaging (FL)
5. DIC Nomarsky
Illumination Guide for Transmitted & Reflected Light
Transparent Specimens – Bacteria, spermatozoa, cells in glass containers, protozoa, mites, fibers, etc.
Light Scattering Objects – Diatoms, fibers, hair, fresh water microorganisms, radiolarians, etc
Light Reflecting Specimens –
Colloidal suspensions, powders and minerals, liquids
Amplitude Specimens –
Stained Tissue, naturally colored specimens, hair & fibers, insects and marine algae
Fluorescent Specimens –
Cells in tissue culture, fluorochrome-stained sections, smears and spreads
Birefringent Specimens –
Mineral thin sections, liquid crystals, melted and re-crystallized chemicals, hairs & fibers, bones & feathers
Specular (Reflecting) Surfaces –
Thin film, mirrors, polished metallurgical samples, integrated circuits
Diffuse (Non-Reflecting) Surfaces – Thick and thin films, rocks & minerals, hair, fiber, bone and insects
Amplitude Surface Features –
Dyed fibers, diffuse metallic specimens, composite materials, polymers
Birefringent Specimens – Mineral thin sections, hair & fibers, bones & feathers, single crystals, oriented films
Fluorescent Specimens –
Mounted cells, fluorochrome-stained sections, smears and spreads