BIM410RPC/FL Hoffman/Relief Phase Contrast & Fluorescence Inverted Biological Microscope


– Inverted Biological Microscope

– Brightfield, and optional Hoffman Modulation Contrast/Relief Contrast and/or Fluorescence

– 4x, 10x, 20x, 40x objective lenses

– 10x/22 eyepieces, 40x-400x total magnifications

– Optional C-Mount Adapters: 0.35x, 0.5x, 0.65x or 1x

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Introduction to Hoffman / Relief Contrast

Relief Contrast is exactly Hoffman Modulation Contrast (HMC), with a minor difference that Zeiss and Leica uses Hoffman Modulation Contrast (HMC) term but Olympus calls is Relief Contrast (RC) and Nikon names it Advanced Modulation Contrast (AMC). Regardless of the name, this is an imaging technique for enhancing the contrast. Like normal phase contrast imaging a biological specimen does not required any staining. Robert Hoffman originally invented the technique in 1975. Like differential interference contrast (DIC) technique, contrast is increased by using components in the light path that convert phase gradients in the specimen into differences in light intensity that are rendered in an image that appears three-dimensional (3D). The 3D appearance of a specimen may often be misleading because a feature that appears to cast a shadow may not necessarily have a distinct physical geometry corresponding to the shadow. HMC systems typically consist of a condenser with a slit aperture, an objective lens with a slit aperture, and a polariser that is fitted between the condenser and the illumination source and is used to control the degree of contrast.

The Relief Contrast or Hoffman Contrast technique is particularly suitable for optical sectioning at lower magnifications. Max 40x magnification is available in the market.

An example of the use of HMC illumination is in in-vitro fertilization, where under brightfield illumination the near-transparent oocyte is hard to see clearly. Reference: Three-dimensional culture of human meniscal cells: Extracellular matrix and proteoglycan production, Helen E Gruber, David Mauerhan, Yin Chow, Jane A Ingram, H James Norton, Edward N Hanley and Yubo Sun, BMC Biotechnology, 2008, 8:54, doi:10.1186/1472-6750-8-54

 

Normal Phase Contrast

Relief /Hoffman Contrast

Features

-Hoffman/ Relief Contrast Module

  • Excellent Choice for Cell Culture Imaging

BIM410RPC/FL Inverted Biological Microscope With Normal Phase Contrast, Hoffman/Relief Phase Contrast and Fluorescence

Brightfield and ContrastWith Fluorescence Imaging

BIM410 is an inverted biological microscope with multiple imaging capabilities, This includes brightfield, normal phase contrast, Olympus Relief Contrast, Fluorescence and Polarized imaging.The default lenses are 4x, 10x, 20x and 40x plan achromat.

 

Relief Contrast / Hoffman Modulation Contrast

The BIM410HMC microscope uses a special slider in the light path and relief contrast objective lenses. The slider is offered in two types,; one for 4x obj lens and another for 10x, 2ox and 40x.

To give you an idea of the image  contrast difference between normal phase contrast and relief contrast, please check the below image. Detailed sample images suing Hoffman modulation contrast technique are shown in the “Images/Videos”tab.

Technical Specifications

1A. BIM410FL

BIM410FL Fluorescence Microscope
EyepieceHigh point eyepiece, wide-field plan eyepiece PL10X/22mm, diopter adjustment : 2 pcs
Objective

Lenses

Infinity long working distance plan fluorescent objective LWDPL4X/0.13/WD18.52mm
Infinity long working distance plan fluorescent objective LWDPL10X/0.3WD7.11mm
Long working distance infinity plan semi-apochromatic phase contrast fluorescent objective. LWDPL20X/0.45WD5.91mm
Long working distance infinity plan semi-apochromatic phase contrast fluorescent objective. LWDPL40X/0.65/WD1.61mm
Stand45° inclined trinocular head, 360° rotatable, interpupillary distance 50-75mm, Splitting ratio for Binocular : Trinocular = 100:0 or 0:100, Reflection and Transmission frame, Coaxial focus system with tension adjustment, quintuple nosepiece, coarse range: 9mm, coke-oriented 6.5mm, 2.5mm down, fine precision: 0.002mm; fixed stage size: 215mmX250mm; Three-band Filter switching mechanism. External wide voltage transformer, input 100-240V, output 12V5A; 5W LED illumination and intensity adjustable;with intelligent ECO infrared sensing system.
N.A.0.3 condenser, working distance 72mm, dismountable
CondenserCentre-adjustable phase contrast slide plate for N.A.0.3,with 2 handle
AttachmentCentering telescop φ30
Glass plate
Metal plate (kidney hole)
Mechanical mobile ruler, moving range120(X)*78(Y)mm
Stage extension plate
φ35 Petri dish holder
Slides holder (φ54 and 26.5×76.5)
Teraseki holder (φ65 and 56X81.5)
LBD color temperature change filter(φ45mm)
IF550 green interference filter(φ45mm)
Fluorescence deviceLED fluorescence lamp house, Four-bands filter sets, 385nm LED, 470nm LED, 560nm LED inside
B1 Filter (470nm LED)
G1 Filter (560nm LED)
UV1 Filter (385nm LED) for option
OthersSpanner
PackingSpange+carton
BIM410FL Optional Accessory
EyepieceHigh point eyepiece, wide-field plan eyepiece PL10X/22mm, diopter
BIM410-10x22E
High point eyepiece, wide-field plan eyepiece PL10X/22mm, diopter adjustment, with reticle
BIM410-10x22R
High point eyepiece, wide-field plan eyepiece PL15X/16mm
BIM410-15×16
ObjectiveInfinity long working distance plan fluorescent objective LWDPL4X/0.13/WD18.52mm
BIM410-4xF
Infinity long working distance plan fluorescent objective LWDPL10X/0.3/WD7.11mm
BIM410-10xF
Infinity long working distance plan fluorescent objective LWDPL20X/0.45/WD5.91mm
BIM410-20xF
Infinity long working distance plan fluorescent objective LWDPL40X/0.65/WD1.61mm
BIM410-40xF
Infinity long working distance plan fluorescent objective LWDPL60X/0.75/WD1.04mm
BIM410-60xF
Long working distance infinity plan semi-apochromatic phase contrast fluorescent objective. LWDPL20X/0.45WD5.91mm
BIM410-20xLWDF
Long working distance infinity plan semi-apochromatic phase contrast fluorescent objective. LWDPL40X/0.65/WD1.61mm
BIM410-40xLWDF

1B. BIM410BF/Ph

Specification
BIM410BF Inverted Microscope
Optical SystemInfinity Color Corrected Optical System
HeadTrinocular Head, Split Ratio 50:50, 45° Inclined, 360° Rotatable, IPD 50-75mm,
EyepiecePlan WF10x/22mm, Diopter Adjustable, High Eye-point,
Objective

Infinity LWD Plan Achromatic Objective 4x/10x/40x

Infinity LWD Positive Phase Contrast Objective 20x
Focus System

Coaxial Focus System With Tension Adjustment, Coarse Range: 9mm, Fine Precision 2μm,

Nosepiece

Quadruple Nosepiece

CondenserLWD N.A. 0.30, W.D. 72mm, Undetachable, Phase Contrast Slide, Centering Telescope
StageRight Hand Controls, Low Positioned, Fixed Mechanical stage, Size 215x250mm, φ35 Petri Dish Holder, Metal Plate And Glass Plate
Light SourceLED Lamp, 5W
Light Indicator
Brightness Adjustable
ECO Infrared Induction System (Auto on/off)
External Wide Voltage Transformer, 100V-240V Input, 12V5A Output,
FilterLBD color temperature change filter(φ45mm)
IF550 green interference filter(φ45mm)
Optional Accessories
Item No.
EyepiecePlan WF15x/16mm, Diopter Adjustable, High Eye-pointBIM410-15xE
StageMechanical mobile ruler, moving range120(X)*78(Y)mm Stage extension plateBIm410-XY
Adapter035x C-MountBIM410-0.35xCA

0.5x C-Mount

BIM410-0.5xCA
0.65x C-MountBIM410-0.65xCA
1x C-MountBIM410-1.0xCA

 

2A. Dimensions of BIM410FL

2B. Dimensions of BIM410BF or BIM410HMC

System Componets

System Configurations

 

Normal Phase Contrast

Relief /Hoffman Contrast

The following images are captured at different magnifications (4x to 40x) using Relief/Hoffman Contrast Module.

 

 

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