Live Cell Imaging Selection Questionnaire

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This list of questions help us to understand your requirements better. Please fill out as much as you can.

Completion of this list prior to contacting BIOIMAGER will optimize the exchange of technical information and help us to serve you better and save your time. Please copy and email or fax this report to BIOIMAGER. E: [email protected], F: (866) 776-2539.

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First Name (required)	Surname (required)
Organization (required)	Department
Position	Office Phone
Lab Phone No	Cellphone/Direct
Email	CC
Address	City
Zip / Postal code	State / Province
Country
1. What is/are the brand & model of your microscope(s)?
2. What is the type of microscope? UprightInvertedStereoOther
3. What is the brand of the XY stage? ASICarl ZeissDelta VisionLeicaLudlMad City LabsMarzhouserNikonOlympusPriorPIOther
4. What Imaging mode or combination of modes will be used? BrightfieldDarkfieldPhase ContrastDICPolarizationModulation ContrastReflection InterferenceFluorescenceMulti-PhotonTIRFVARELConfocal
5. What objective magnifications are you using? (Please include all that apply) 1.25x2x2.5x4x5x10x20x25x40x50x60x80x100x
Any Oil Immersion Lens?
Any water immersion lens?
6. What is the numerical aperture (NA) and working distance (WD) of the condenser?
7.1 How long do the cells need to be maintained on the microscope (minutes, days, or weeks)?
7.2 What is the time interval between images (seconds, minutes, or days)?
8. What is/are the specimen type? Please select all apply. Adherent monolayerCell suspensionNatural tissueArtificial membraneMolecular ImagingOther
9. Describe the experiment as it relates to the microscopy imaging. (specimen, object of experiment and imaging protocol)
10. What are the types of samples: (Please include all that apply) well-plateglass-slide chambers55-mm petri-dishes35-mm petri-dishes, please specify the brand NancCorningWillcoOthers
11. What temperature do the specimens need to be maintained?
12. Do you need both Cooling & heating system or heating only?
13. What is the appropriate chamber type? OpenClosedUnsure
14. Will micromanipulators be used and when? NoneBefore imagingDuring imagingBefore & During
15. Does the specimen need perfusion of media? YesNo If yes, please include flow rates or volume exchange rate Intermittent (manual)AutomatedContinuousSingle or multiple perfusate sources
16. Do you need the temperature of your objective lenses to be controlled?
17. Do you need a gas (CO2/O2/N2) mixer? If yes, what is the condition(s)?
18. What is the budget range for this project?

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