BL240 is a complete, turn-key system for quantitative fluorescence imaging of small animals in vivo. The imaging approach is non-invasive and high resolution and uses high-power LEDs. It provides very large FOV with 2x to 40x objective lenses, and has LEDs and filters from UV to far red.
One of our clients in North Western University (Chicago, IL, USA) wrote us: “I am looking for the ability to observe fluorescent cello behaviors in mouse brain with cranial window installations. Ideally, I can see eGFP, mKate2 [Far Red, EX:588nm, EM:633], and cerulean [EX:433nm, EM: 475nm] labeled stem cells in brain tumor xenografts under the cranial window cover glass. Blood vessels will be counterstained with far red fluorophores, so 4 color cubes distinguishing these colors would be ideal. Since we do have multiphoton confocal microscopy at the NU core, we do use it for some live animal imaging, but there is requirements on the fluorophores and only 20x waterline available. I would like the 8x zoom you have for greater detail and also larger field of views.
What software and camera resolution would be used for the image acquisition?
Fig. 8b, c, g, h are the ideal FOV and resolution if we can achieve this, 75% of the images will be collected at this level of magnification/FOV/detail. I would love to collect images that are 4X more in magnification, but if the distance is an issue, then I can sacrifice. In order to keep the mouse head steady during anesthesia (to avoid position changes due to breathing/waking etc.), I have a stereotaxic frame to keep the mouse stable, shown here in the model and brand: Stoelting 51625.
The mouse skull will be the focus, and it is usually 1.5cm additional height from the top of the stereotaxic frame platform, which itself is 4.25” tall per product spec of 51625. So, the clearance between lens and the microscope bottom should be between 10-12cm. Solution:
We prepared this package of BL240 which has LED fluorescent attachment with UV, Blue, Green and Far Red LEDs and its relevant filters, used 2x, 4x, 5x, 10x, 20x and 40x obj lenses with a camera and proper c-mount adapter which gives the 7mm x6mm FOV at 2x obj lenses while he can also ge high resolution images at micron level using 40x plan fluorescence lens. In addition we customized the microscope stand which allows a large sample height up to 100mm, a fine/coarse focus and a great XY stage to easily move the sample (a live mouse mounted on a stereotaxic).
In addition, the price of the package was way economic and the images were extremely satisfactory. Just read in the customer reviews what he thought about this.
LED fluorescent microscope
2x,4x, 5x, 10x, 20x and 40x objective lenses
Observation from micro to macro level.
super large sample height is allowed (100mm)
FOV can be as large as 6x7mm at 2x and at few hundred microns with 40x lens.
LEDs and filters from UV to far red to use with Dapi/Hoechst staining, GFP/AlexaFluor 488, RFP/Alexa 555 and Alexa 647 fluorophores.
very economic solution
In Vivo Imaging Solutions
BL240 is a complete, turn-key system for quantitative fluorescence imaging of small animals in vivo, offered by Bioimager. The imaging approach is non-invasive and high resolution. The package is offered mainly for a big fraction of the body imaging and single cell level imaging for small animals such as mice, rat, zebrafish, chicken, etc. These packages have the lowest price and the best performance per price in the market. Please contact us for customization and free consultation.
* Ideal Screening Fluorescence Microscope
* We offer a low-price solution for stereo fluorescence imaging, screening and manipulation of transgenic species.
* Long working distance
* Base configurations available for fluorescence only or with LED transmitted light source.
* Bright, easily visible outputs. Multi-wavelength excitation.
* Custom light adapter source, upgradable for bright GFP or any custom design.
* Perfect tool for Zebrafish, embryo development studies, and Vet Labs.
* #1 choice for C. Elegans fluorescent Imaging, macroscopic pictures of bacterial colonies, and microfluidic chips.
* Tens of accessories and optional items to complete your imaging need
* We offer up to 40 different specialized filter cubes.Please contact us for the listing.
Trinocular head, inclination of 22.5°,diopter adjustment 5,
Wide field WF10X (field number: Φ22mm)
Infinity Corrected Objective Lenses
BIMT410-2.0x Plan Achromatic Obj lens 2x/ NA 0.05, WD 7.20mm
BUM5-4xF Plan Fluor Obj lens 4x/NA 0.13
PlanFluor 5X Plan Fluor Objective 5X/NA 0.15/W.D 20mm
BUM5-10xF PlanFluor 10X Objective
BUM5-20xF Plan Fluor 20X objective lens
BIM5-40xF Plan Fluor Objective 40X obj lens
Coaxial coarse/fine focus system, with coarse focus knob tension adjustable device, minimum division of fine focusing: 2μm. Max. height of sample: 100mm. Range: 35mm