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High-Resolution & High-Power Fluorescence In Vivo Imaging

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BL240 is a complete, turn-key system for quantitative fluorescence imaging of small animals in vivo. The imaging approach is non-invasive and high resolution and uses high-power LEDs. It provides very large FOV with 2x to 40x objective lenses, and has LEDs and filters from UV to far red.

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$15,888.00 $20,000.00

Client Story:
One of our clients in North Western University (Chicago, IL, USA) wrote us: “I am looking for the ability to observe fluorescent cello behaviors in mouse brain with cranial window installations. Ideally, I can see eGFP, mKate2 [Far Red, EX:588nm, EM:633], and cerulean [EX:433nm, EM: 475nm] labeled stem cells in brain tumor xenografts under the cranial window cover glass. Blood vessels will be counterstained with far red fluorophores, so 4 color cubes distinguishing these colors would be ideal. Since we do have multiphoton confocal microscopy at the NU core, we do use it for some live animal imaging, but there is requirements on the fluorophores and only 20x waterline available. I would like the 8x zoom you have for greater detail and also larger field of views.

What software and camera resolution would be used for the image acquisition?

Later he added: …The FOV for light microscopy should be capable of this nature protocol article (Goldey et al., Nature Protocols 9, 2515–2538,2014).

Fig. 8b, c, g, h are the ideal FOV and resolution if we can achieve this, 75% of the images will be collected at this level of magnification/FOV/detail. I would love to collect images that are 4X more in magnification, but if the distance is an issue, then I can sacrifice. In order to keep the mouse head steady during anesthesia (to avoid position changes due to breathing/waking etc.), I have a stereotaxic frame to keep the mouse stable, shown here in the model and brand: Stoelting 51625.

The mouse skull will be the focus, and it is usually 1.5cm additional height from the top of the stereotaxic frame platform, which itself is 4.25” tall per product spec of 51625. So, the clearance between lens and the microscope bottom should be between 10-12cm.
Solution:
We prepared this package of BL240 which has LED fluorescent attachment with UV, Blue, Green and Far Red LEDs and its relevant filters, used 2x, 4x, 5x, 10x, 20x and 40x obj lenses with a camera and proper c-mount adapter which gives the 7mm x6mm FOV at 2x obj lenses while he can also ge high resolution images at micron level using 40x plan fluorescence lens. In addition we customized the microscope stand which allows a large sample height up to 100mm, a fine/coarse focus and a great XY stage to easily move the sample (a live mouse mounted on a stereotaxic).

In addition, the price of the package was way economic and the images were extremely  satisfactory. Just read in the review what he thought about this.

  • LED fluorescent microscope
  • 2x,4x, 5x, 10x, 20x and 40x objective lenses
  • Observation from micro to macro level.
  • super large sample height is allowed (100mm)
  • FOV can be as large as 6x7mm at 2x and at few hundred microns with 40x lens.
  • LEDs and filters from UV to far red to use with Dapi/Hoechst staining, GFP/AlexaFluor 488, RFP/Alexa 555 and Alexa 647 fluorophores.
  • fine/coarse focus
  • very economic solution

In Vivo Imaging Solutions

High-Resolution & High-Power Fluorescence In Vivo Imaging-10308

BL240 is a complete, turn-key system for quantitative fluorescence imaging of small animals in vivo, offered by Bioimager. The imaging approach is non-invasive and high resolution. The package is offered mainly for a big fraction of the body imaging and single cell level imaging for small animals such as mice, rat, zebrafish, chicken, etc. These packages have the lowest price and the best performance per price in the market. Please contact us for customization and free consultation.

* Ideal Screening Fluorescence Microscope
* We offer a low-price solution for stereo fluorescence imaging, screening and manipulation of transgenic species.
* Long working distance
* Base configurations available for fluorescence only or with LED transmitted light source.
* Bright, easily visible outputs. Multi-wavelength excitation.
* Custom light adapter source, upgradable for bright GFP or any custom design.

* Perfect tool for Zebrafish, embryo development studies, and Vet Labs.

* #1 choice for C. Elegans fluorescent Imaging, macroscopic pictures of bacterial colonies, and microfluidic chips.

* Tens of accessories and optional items to complete your imaging need

* We offer up to 40 different specialized filter cubes.Please contact us for the listing.

Brief Specifications

HeadTrinocular head, inclination of 22.5°,diopter adjustment 5,

interpupillary 53mm-75mm

EyepieceWide field WF10X (field number: Φ22mm)
Infinity Corrected Objective LensesBIMT410-2.0x Plan Achromatic Obj lens 2x/ NA 0.05, WD 7.20mm
BUM5-4xF Plan Fluor Obj lens 4x/NA 0.13
PlanFluor 5X Plan Fluor Objective 5X/NA 0.15/W.D 20mm
BUM5-10xF PlanFluor 10X Objective
BUM5-20xF Plan Fluor 20X objective lens
BIM5-40xF Plan Fluor Objective 40X obj lens
Focus SystemCoaxial coarse/fine focus system, with coarse focus knob tension adjustable device, minimum division of fine focusing: 2μm. Max. height of sample: 100mm. Range: 35mm
NosepieceQuintuple nosepiece, RMS Thread.
StageBase overall size:300mmX250mm
Mechanical stage overall size:160mmX140mm, moving range:30mmx35mm

 

1. BMV220 Microscope

HeadTrinocular head, inclination of 22.5°,diopter adjustment 5,

interpupillary 53mm-75mm

EyepieceWide field WF10X (field number: Φ22mm)
Infinity Corrected Objective LensesBIMT410-2.0x Plan Achromatic Obj lens 2x/ NA 0.05, WD 7.20mm
BUM5-4xF Plan Fluor Obj lens 4x/NA 0.13
PlanFluor 5X Plan Fluor Objective 5X/NA 0.15/W.D 20mm
BUM5-10xF PlanFluor 10X Objective
BUM5-20xF PlanFluor 20X objective lens
BIM5-40xF Plan Fluor Objective 40X obj lens
Focus SystemCoaxial coarse/fine focus system, with coarse focus knob tension adjustable device, minimum division of fine focusing: 2μm. Max. hight of sample: 100mm. Range: 35mm
NosepieceQuintuple nosepiece, RMS Thread.
StageBase overall size:300mmX250mm
Mechanical stage overall size:160mmX140mm, moving range:30mmx35mm

2. Sample Holder: stereotaxic frame Stoelting 51625 or similar model (Optional)

Overall Dimensions: 10 3/4″L x 2 3/4″W x 4 1/4″H

 

3. FL-BUM36LED-4 Customized 4-Channel LED Epi-Fluor Attachment

SAMSUNG CSC

LEDFluorophoresFilters
NameEXNameEXEMEXDMEM
Ultraviolet  (UV)365nmDapi / Hoechst350 nm470 nm330-385nm400420-470nm
Blue light (B)470nmFITC / GFP490 nm525 nm460nm-495nm505510-550
Green light (G)505nmAlexa Fluor 555555 nm580 nm460nm-550nm570590-650
Red (R)625nmAlexafluor 647650 nm665 nm600-660nm660660nm

 

4.BIC-T3C USB 3.0 HD Retina Colorful CMOS Microscope Camera

ModelBIC-T3C-2C2
SensorIMX185 Color

1/1.9″, 7.20mm x 4.5mm

Pixel2.3MP or 1920×1200

3.75 μm/pixel

Speed[email protected]×1200;

[email protected]×600

G Sensitivity

Dark Signal

1120mv with 1/30s

0.5mv with 1/30s

Binning1×1, 2×2
Exposure time0.244ms~2000ms
InterfaceUSB 3.0

5. Sealable dark room (optional)

Package Contents

#ProductQT
BMV27FLED Customized fluorescence laboratory microscope for imaging of stem cells in mice brain tumor xenografts under the cranial window cover glass, and is composed of the following items:1
1BUM27C upright microscope frame with Trinocular head, EW10x eyepieces, with custom mount of the client sample holder1
2FL-BUM500LED-4 4-channel LED Epi-fluorescent attachment with customized couplers, and without filters1
3Dapi Filter and LED1
4FITC Filter and LED1
5Alexa Fluor 555 Filter and LED1
6Alexafluor 647 Filter and LED1
7BIMT410-2.0x Plan Achromatic Obj lens 2.0x/ NA 0.05, WD 7.20mm1
8BUM5-4xF Plan Fluor 4x/0.131
9PlanFluor 5X Plan Semi Apochromat Objective 5X/NA 0.15/W.D 20mm1
10BUM5-10xF PlanFluor 10XObjective1
11BUM5-20xF PlanFluor 20Xobjective lens1
12BIM5-40xF Plan Fluor Objective 40X obj lens1
13BIC-T3C-2C2 Retina 2.3MP (1920×1200) 1/1.9” USB3.0 Colorful Digital CMOS Camera.1
14BUM5-0.5xC 0.5x C-mount adapter with adjustable focus1
15BIA-CA0.1 0.01 mm calibration slide, English and metrics system.1
16BIA-W1 1 year warranty1
17CSH Customization, Shipping and Handling1
 
***OPTIONAL ITEMS***
18BUM5-15xE16 WF15x/16mm for BUM500 microscope2
19BI830-2.5x Plan Semi-Apo BF Obj lens 2.5x/ NA 0.07, WD 5.7mm1
20PlanFluor 50X Plan Semi Apochromat Objective 50X/NA 0.80/W.D 1mm **RECOMMENDED**1
21BIC-T3D-1.4C 1.4MP (1360×1024) 2/3” USB3.0 Colorful Digital CMOS Camera. **RECOMMENDED**1
22BRC-1600 Retina 16MP (4608×3456) 1/2.33” USB3.0 Colorful Digital CMOS Camera.1
23BUM5-0.75xCV 0.75x adjustable focus c-mount adapter1
24BIA-W2 Extended warranty for 2 years (including the first year)1

5x obj lens, mice brain, Alexa 555 staining; Green LED, Red EM

 

20x obj lens, Alexa 555 staining

 

Basics and Principles of Fluorescence Microscopes

Live Cell Imaging

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  1. younes

    One of the clients for this custom package was Dr. Dou Yu at Massachusetts Institute of Technology (MA, USA). He left this feedback in google business review:
    “Absolutely the best vendor for custom microscopy needs in the scientific research areas! Extremely helpful, considerate, professional, and highly reliable in communication, delivery, and follow up! Do yourself a favor and talk to Dr. Younes Leysi, you will be happy that you can do great work with much more reasonable budget!!!”.               

    younes

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