Save $USD 1,500

BUM500DFL Digital Darkfield Microscope

  • BUM500 Series biological Upright Microscope
  • Brightfield and darkfield imaging with optional Phase-Contrast, Polarizing, Epi-fluor imaging
  • 4x, 10x, 40x, 60x and 100x Lenses included, optional 2x, 2.5x, 5x, 20x, 50x, 150x
  • High level microscope, designed for laboratory study in darkfield mode
  • Includes a retina display camera (6MP HD Camera & 11.6″ Retina Monitor)
  • Comes with great darkfield kit

$USD 4,500.00 $USD 6,000.00


BUM500 Biological microscope with trinocular head 4x, 10x, 40x, 60x and 100x BF and DF obj lenses brightfield and darkfield condensers 10x and 20x eyepieces, 2000x total magnifications
c-mount adapters which gives up to 2000x magnification of optical images
BRC-6012 Retina display camera 6MP High Definition Camera 11.6″ Retina Display Live 60 fps via HDMI cable and 30fps speed via USB
professional darkfield kit for professional users


Newly designed with comfortable operating and ergonomic design, better illumination, convenient replacement of attachment with sliding-in center adjustable condenser. –Infinity Optical System Supply High Quality Optical Image –Eyepiece EW10x/22mm, Eyepiece Tube Dia.30mm –Swing-out Condenser N.A.0.95/0.25 –External Illumination, Aspherical Collector, Halogen Lamp 6V30W –Full Line of Dark Field, Phase Contrast, Polarizing, Fluorescent Attachment as well as automatic XY or Z focus is available for upgrade

Using Oil for oil condenser and oil immersion objective lenses:

Darkfield Imaging Principles

Using  a darkfield microscopy, we see some details that we could not observe using brightfield technique. To know the main difference between bright-field an dark-field , think of seeing some fogs an drain drops under light shine. That is a natural darkfield imaging.

Darkfield objective lenses and condensers are the core elements of the darkfield imaging:

Description of the microscope

This microscope is an ideal instrument in biological, histological, pathological, bacteriology, immunizations and pharmacy field when the darkfield imaging is matter. It is an excellent choice for live and dead blood cells analysis.This microscope is a high level microscope which is specially designed for laboratory study. It adopts an Infinite optical system, reasonable structure and ergonomic design and comes with darkfield kit. With an innovative optical and structure design idea, excellent optical performance and easy to operate system, this laboratory biological microscope makes your laboratory works enjoyable.
BUM500DIC Upright Biological Microscope with DIC and Phase Contrast Imaging-0


1. Microscope

Optical SystemInfinite Optical System
Viewing HeadSwiveling Siedentopf type Trinocular Head, Inclined at 30°, Interpupillary 48-75mm
Angle Adjustable Binocular/ Trinocular Head
EyepieceExtra Wide Field Eyepiece EW10×/ 22
NosepieceBackward Quintuple Nosepiece
Backward Sextuple Nosepiece
ObjectiveInfinite Plane Achromatic Objective 4×, 10×, 40×, 100×
Infinite Plane Achromatic Objective 20×
CondenserSwing-out Condenser NA 0.9/ 0.25
StageDouble Layers Mechanical Stage 185×142mm, moving range 75×55mm
IlluminationExternal Illumination, Aspherical Collector, Halogen Lamp 6V/30W
External Illumination, Aspherical Collector, Halogen Lamp 24V/100W
Video AdapterC Mount 1×
C Mount 0.5×
FilterBlue Filter
Green Filter
Multi Viewing AttachmentsMulti Viewing Attachments for 5 persons
Multi Viewing Attachments for 3 persons
Multi Viewing Attachments for 2 persons
Dark Field AttachmentDark-field Condenser(Dry)
Dark-field Condenser(Oil)
Optional AttachmentPhoto Attachment
Polarization Attachment
Turret Phase Contrast Kit
Epi-fluorescence Attachment
Temperature Controlled Stage
Dimension& G. W.43.5cm*39.5cm*59cm, 14kg

Note: ● Standard Outfit, ○ Optional

2. BRC-6012 Retina Display Camera

Sensor size1/2.7″ (assemble with Exfocus05 to 80% field of view)
Live Resolution1920×1080
Resolution2MP Effective pixels; via USB
6MP with pixel shifting; via HDMI, USB, SD card
File formatHDMI mode: JPG, AVI USB mode: JPG, AVI, TIF, PNG.
Magnification rate1X~10X
Frame speed1920×1080 30fps via USB2.0 HDMI
1920×1080 60fps via USB2.0 HDMI
Image Record Video RecordHigh speed SD Card (8 GB)
1080p 30fps in SD Card
1080p 30fps in PC
Exposure time0.001 sec ~ 10.0 sec
White balanceAuto
SettingsGain, Gamma, Saturation, Contraction
Built- in softwareCloud 1.0 ver
PC softwareiSCapture
Output model 1USB2.0
Output model 2HDMI
System CompatibleWindows XP, 7/8; 32/64bit

Package Contents

BUM500DF biological upright laboratory microscope customized for live blood cell imaging and is composed of the following items:1
1BUM500 upright microscope with Trinocular head, EW10x, Quintuple, Infinity Plan Objective 4x, 10x, 40x, Mechanical Stage, Swing out NA0.9-0.25 condenser, Coaxial Coarse & Fine, Halogen 6V30W Koehler Illumination.1
2BUM5CD0.95D Darkfield condenser, dry, N.A. 0.7-0.95 Plan Apo for BUM500 microscope1
3BUM5-60xDF 60x(S) brightfield / darkfield for BUM500DF microscope1
4BUM5-100X-DF Infinite Plan 100X with Iris Diaphragm for Oil Dark Field Observation using BUM500DF microscope1
5BUM5CD1.25O Darkfield condenser, Oil, N.A 1.25-1.36 Plan Apo for BUM500 microscope1
6BRC-6012 Retina Display Camera with 11.6” Retina HD Monitor, 6MP built-in Camera, 8GB SD, image acquisition Software.1
7BUM5-0.37xC 0.37x C-mount adapter1
8BUM5-1xC 1x C-Mount adapter1
9BIA-W1 1 year warranty1
11BUM5-15xE16 WF15x/16mm for BUM500 microscope ***recommended***2
12BUM500-100WLH Upgrade to 100W lamp-house for BUM500DF microscope ***recommended***1
13BRC-1600 Retina 16MP (4608×3456) 1/2.33” USB3.0 Colorful Digital CMOS Camera.1
14BUM5-0.37xCV 0.37x adjustable focus c-mount adapter1
15BUM5-0.5xCV 0.5x adjustable focus c-mount adapter1
16BUM5-0.75xCV 0.75x adjustable focus c-mount adapter1
17BIA-0.1CS Calibration slide ***recommended***1
18BIA-W2 Extended warranty for 2 years (including the first year)1

System Diagram

This system can be upgraded to a fully automated model. Please check Other models of this microscope: BUM500Fl Epi-Fluor Upright Microscope BUM500A Automatic Biological Microscope BMM500 Metallurgical Upright Microscope

Video 1. Phase Contrast Kit and Sample Images

BUM-PHK Phase Contrast Kit for Upright Biological Microscopes is composed of 10x, 20x, 40x , 60x and 100x (oil) Phase contrast objective lenses, green filter, Phase alignment tool and turret condenser with different annulus.
In this video we look at the samples images of an Onion Skin thins section slide and Tilia (basswood) thin section slide, using this kit installed on Bioimager BUM800FL upright biological microscope and connected a 2.3MP USB3.0 CMOS Colorful Camera as well as a 0.5x C-Mount Adapter.

4x 10x darkfield 10x 40x dark-field 40x 60x darkfield 100 100x lens (dry) darkfield 100x 60x darkfield 100x 100x (oil) lens with iris, darkfield   brightfield images

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