BUM500FL Fluorescence microscope is mainly used to study the absorbing, transportation, chemicals distribution and positioning in cells. It is widely used in universities, hospitals and life science labs for disease examination, immune diagnosis and scientific research.
BUM500FL upright fluorescence microscope is a laboratory fluorescent microscope with great infinity corrected optical system. The microscope uses 100W mercury lamp as the light source, the fluorescent filter wheel has 6 positions for filter blocks, which allows easy switch of filter blocks for various fluorochrome.
BUM500FL Upright Fluorescence Microscope
BUM500FL upright fluorescence microscope uses HBO / Mercury as the light source and has infinity corrected plan Achromat objective lenses of 4x, 10x, 40x, 100x and optional plan fluor objective lenses. The microscope includes B and G filters by default which are great for GFP / Alexa Fluor 488 and RFP / Texas Reed / mCherery /tdTomato stainings. In addition UV and V filters can be included for Dapi/Hoechst stainings. BIOIMAGER offers 40 Custom filters.
You can add phase contrast imaging kit and simple polarizer as well.
- Great image quality with infinity corrected optical system and Koehler illumination
- 100W wide-spectrum HBO/Mercury bulb
- Six-position filter wheel with pre-installed B and G filters.
- Reversed quintuple nosepiece and optional sextuple
- Four plan-achromatic objective lenses: 4X, 10X, 40X, 100X(oil)
- High resolution fluorescent objective lenses
- Advanced and precision lamp housing reduces the light leak.
- Reliable power supply with digital display and timer.
- Compensation-free Siedentopf interpupillary adjustment
BUM500FL Fluorescence Microscope
designed at 2010 with comfortable operating, ergonomic structure, better illumination, and convenient replacement of attachment with sliding-in center adjustable condenser.
–Infinity Optical System Supply High Quality Optical Image
–Eyepiece EW10x/22mm, Eyepiece Tube Dia.30mm
–Swing Condenser N.A.0.95/0.25
–External Illumination, Asperical Collector, Halogen Lamp 6V30W
–6 Position Disc Reflected Flourescent System Can Contain 6 Filters
–B,G, U, V and custom Fluorescent Filters Available
–Excellent Fluorescent Image With High Resolution Fluorescent Objectives
–Protect The Light Leak With Advanced Lamp Housing
|BUM500FL Disc Fluorescence Microscope|
|Optical System||Infinity Optical System|
|Head||Seidentopf Trinocular Head, 30° Inclined, Interpupillary 48~75mm, Diopter Adjustable|
|Eyepiece||Extra Wide Field Eyepiece EW10x/22mm, Eyepiece Tube Dia.30mm|
|Nosepiece||Backward Quintuple Nosepiece|
|Objective||Infinity Plan Achromatic Objective 4x, 10x, 40x, 100x|
|Working Stage||Double Layer Mechanical Stage, 185 x 142mm, Moving Range 55 x 75mm|
|Focusing||Coaxial Coarse & Fine Adjustment, Focusing Range 24mm, Fine Division 0.001mm, Coarse Stroke 37.7mm|
|Condenser||Swing Condenser N.A.0.95/0.25|
|Illumination||External Koehler Illumination, Aspherical Collector, Halogen Lamp 6V30W, Brightness Adjustable|
|Fluorescent System||6 Position Disc Reflected Fluorescent System|
–Power Supplier Box, 220V/110V Interchangeable, Digital Display
–100W HBO Ultra Hi-Voltage Spherical Mercury Lamp
–Barrier To Resist Ultraviolet Light
–Fluorescent Free Oil
–Blue Excitation Filter, BP460~490, DM500, BA520
–Green Excitation Filter, BP510~550, DM570, BA590
BUM500FL Disc Fluorescence Microscope Optional Accessories
|Objective||Infinity Plan Achromatic Objective 20x||BUM5-20x|
|Fluorescent System||Infinity Plan Fluorescent Objective||4x/0.13/0.17, W.D.16.3||BUM5F-4xF|
|Blue Excitation (B1) Filter, BP460~495, DM505, BA510~550||BUM5F-B1|
|Ultraviolet Excitation Filter, BP330~385, DM400, BA420||BUM5F-U|
|Violet Excitation Filter, BP400~410, DM455, BA455||BUM5F-V|
|Netural Filter ND6||BUM5-ND6|
|Netural Filter ND25||BUM5F-ND25|
|Epi-Fluorescent Centering Slide||BIA-F-L-OL|
|Optical System||Infinite Optical System||●|
|Viewing Head||Seidentopf Trinocular Head, Inclined at 30°, Interpupillary Distance 48-75mm||●|
|Eyepiece||Extra Wide Field Eyepiece EW10×/22mm, Eyepiece tube diameter 30mm||●|
|Nosepiece||Backward Quintuple Nosepiece||●|
|Backward Sextuple Nosepiece||○|
|Objective||Infinite Plan Achromatic Objective||2×/0.05, W.D.=18.3mm||○|
|100×/1.25(S, Oil), W.D.=0.13mm||●|
|Infinite Plan Fluorescent Objective||4×/0.13, W.D.=16.3mm||○|
|100×/1.3(S, Oil), W.D.=0.13mm||○|
|Condenser||Swing Condenser NA 0.9/ 0.25||●|
|Focusing||Coaxial Coarse and Fine Adjustment, Fine Division 0.001mm, Coarse Stroke 37.7mm per Rotation, Fine Stroke 0.1mm per Rotation, Moving Range 24mm||●|
|Stage||Double Layers Mechanical Stage 185×142mm, Moving Range 75×55mm||●|
|Photo Adapter||Used to connect Nikon or Canon DLSR camera to the microscope||○|
|Video Adapter||1× or 0.5× C-mount adapter||○|
|Transmitted Kohler Illumination||External Illumination, Aspherical Collector with Kohler Illumination, Halogen Lamp 6V/30W, Brightness Adjustable||●|
|External Illumination, Aspherical Collector with Kohler Illumination, Halogen Lamp 24V/100W, Brightness Adjustable||○|
|3W LED Illumination, Brightness Adjustable||○|
|5W LED Illumination, Brightness Adjustable||○|
|Reflected Light Source||Excitation||Dichroic Mirror||Barrier Filter|
|Lamp||100WHBO Ultra Hi-voltage Spherical Mercury Lamp||●|
|Protection barrier||Barrier to Resist the Ultraviolet Light||●|
|Power Supplier||Power Supplier NFP-1, 220V/ 110VVoltage Interchangeable, Digital Display||●|
|Immersion Oil||Fluorescent Free Oil||●|
|Filter||Neutral ND25/ ND6 Filter||○|
Note: ●Standard Outfit, ○Optional
Video 1: Main Body and Performance Overview:
Video 2: Imaging Capability: Brightfield imaging with transmitted or Reflected Light
Video 3. Phase Contrast Kit and Sample Images
BUM-PHK Phase Contrast Kit for Upright Biological Microscopes is composed of 10x, 20x, 40x , 60x and 100x (oil) Phase contrast objective lenses, green filter, Phase alignment tool and turret condenser with different annulus.
In this video we look at the samples images of an Onion Skin thins section slide and Tilia (basswood) thin section slide, using this kit installed on Bioimager BUM800FL upright biological microscope and connected a 2.3MP USB3.0 CMOS Colorful Camera as well as a 0.5x C-Mount Adapter.
Video 4. LED Fluorescent Attachment
This video demonstrates Bioimager BUM360FLED-A LED Fluorescence attachment to use with a standard upright microscope. It comes with 5W LED of Violet (V), Ultraviolet (UV), Blue (B) and Green (G) LEDs, and its correspondent excitation and emission filters. The video demonstrates the optics and mechanics as well as sample images at different magnifications.