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BUM500FL Epi-Fluorescence Upright Microscope

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  • Compound Fluorescence Microscope
  • Blue & Green Filters (for GFP and RFP/Texas Red/mCherry staining) and optional UV (for Dapi/Hoechst) and V filters
  • 4x, 10x, 40x and 100x objective lenses included, optional 2x, 20x, 50x, 60x, 150x.
  • Siedentopf trinocular viewing head, inclined at 30°, interpupillarty distance 48mm-75mm
  • Wide Field Eyepiece WF10×/22
  • Infinite Plan Achromatic Objective 4×, 10×, 40×, 100×
  • Swing Condenser NA 0.9/ 0.25
  • Coaxial Coarse & Fine Adjustment, Fine Division 0.001mm
  • External Illumination, Aspherical Collector, Halogen Lamp 6V/ 30W

In stock

$3,595.00 $7,750.00

Application
BUM500FL Fluorescence microscope is mainly used to study the absorbing, transportation, chemicals distribution and positioning in cells. It is widely used in universities, hospitals and life science labs for disease examination, immune diagnosis and scientific research.
Introduction
BUM500FL upright fluorescence microscope is a laboratory fluorescent microscope with great infinity corrected optical system. The microscope uses 100W mercury lamp as the light source, the fluorescent filter wheel has 6 positions for filter blocks, which allows easy switch of filter blocks for various fluorochrome.


BUM500FL Upright Fluorescence Microscope
BUM500FL upright fluorescence microscope uses HBO / Mercury as the light source and has infinity corrected plan Achromat objective lenses of 4x, 10x, 40x, 100x and optional plan fluor objective lenses. The microscope includes B and G filters by default which are great for GFP / Alexa Fluor 488 and RFP / Texas Reed / mCherery /tdTomato stainings. In addition UV and V filters can be included for Dapi/Hoechst stainings. BIOIMAGER offers 40 Custom filters.

You can add phase contrast imaging kit and simple polarizer as well.

Feature

    1. Great image quality with infinity corrected optical system and Koehler illumination
    2. 100W wide-spectrum HBO/Mercury bulb
    3. Six-position filter wheel with pre-installed B and G filters.
    4. Reversed quintuple nosepiece and optional sextuple
    5. Four plan-achromatic objective lenses: 4X, 10X, 40X, 100X(oil)
    6. High resolution fluorescent objective lenses
    7. Advanced and precision lamp housing reduces the light leak.
    8. Reliable power supply with digital display and timer.
  1. Compensation-free Siedentopf interpupillary adjustment


BUM500FL Fluorescence Microscope

designed at 2010 with comfortable operating, ergonomic structure, better illumination, and convenient replacement of attachment with sliding-in center adjustable condenser.

–Infinity Optical System Supply High Quality Optical Image
–Eyepiece EW10x/22mm, Eyepiece Tube Dia.30mm
–Swing Condenser N.A.0.95/0.25
–External Illumination, Asperical Collector, Halogen Lamp 6V30W
–6 Position Disc Reflected Flourescent System Can Contain 6 Filters
–B,G, U, V and custom Fluorescent Filters Available
–Excellent Fluorescent Image With High Resolution Fluorescent Objectives
–Protect The Light Leak With Advanced Lamp Housing

BUM500FL Disc Fluorescence Microscope
Optical SystemInfinity Optical System
HeadSeidentopf Trinocular Head, 30° Inclined, Interpupillary 48~75mm, Diopter Adjustable
EyepieceExtra Wide Field Eyepiece EW10x/22mm, Eyepiece Tube Dia.30mm
NosepieceBackward Quintuple Nosepiece
ObjectiveInfinity Plan Achromatic Objective 4x, 10x, 40x, 100x
Working StageDouble Layer Mechanical Stage, 185 x 142mm, Moving Range 55 x 75mm
FocusingCoaxial Coarse & Fine Adjustment, Focusing Range 24mm, Fine Division 0.001mm, Coarse Stroke 37.7mm
CondenserSwing Condenser N.A.0.95/0.25
IlluminationExternal Koehler Illumination, Aspherical Collector, Halogen Lamp 6V30W, Brightness Adjustable
Fluorescent System6 Position Disc Reflected Fluorescent System
–Power Supplier Box, 220V/110V Interchangeable, Digital Display
–100W HBO Ultra Hi-Voltage Spherical Mercury Lamp
–Barrier To Resist Ultraviolet Light
–Fluorescent Free Oil
–Blue Excitation Filter, BP460~490, DM500, BA520
–Green Excitation Filter, BP510~550, DM570, BA590

BUM500FL Disc Fluorescence Microscope Optional Accessories

ObjectiveInfinity Plan Achromatic Objective 20xBUM5-20x
Fluorescent SystemInfinity Plan Fluorescent Objective4x/0.13/0.17, W.D.16.3BUM5F-4xF
10x/0.3/0.17, W.D.12.4BUM5F-10xF
20x/0.5/0.17,W.D.1.5BUM5F-20xF
40x/0.75/0.17,W.D.0.35BUM5F-40xF
100x/1.3/0.17,W.D.0.13BUM5F-100xF
Blue Excitation (B1) Filter, BP460~495, DM505, BA510~550BUM5F-B1
Ultraviolet Excitation Filter, BP330~385, DM400, BA420BUM5F-U
Violet Excitation Filter, BP400~410, DM455, BA455BUM5F-V
Netural Filter ND6BUM5-ND6
Netural Filter ND25BUM5F-ND25
Epi-Fluorescent Centering SlideBIA-F-L-OL
AdapterPhoto AdapterBUM5-SLR
C-Mount 1.0xBUM5-1.0xCA
C-Mount 0.5xBUM5-0.5xCA

Specification

ItemSpecificationBUM500FL
Optical SystemInfinite Optical System
Viewing HeadSeidentopf Trinocular Head, Inclined at 30°, Interpupillary Distance 48-75mm
EyepieceExtra Wide Field Eyepiece EW10×/22mm, Eyepiece tube diameter 30mm
NosepieceBackward Quintuple Nosepiece
Backward Sextuple Nosepiece
ObjectiveInfinite Plan Achromatic Objective2×/0.05, W.D.=18.3mm
4×/0.10, W.D.=17.3mm
10×/0.25, W.D.=10mm
20×/0.40, W.D.=5.1mm
40×/0.65(S), W.D.=0.54mm
60×/0.8(S), W.D.=0.14mm
100×/1.25(S, Oil), W.D.=0.13mm
Infinite Plan Fluorescent Objective4×/0.13, W.D.=16.3mm
10×/0.30, W.D.=12.4mm
20×/0.50, W.D.=1.5mm
40×/0.75(S), W.D.=0.35mm
100×/1.3(S, Oil), W.D.=0.13mm
CondenserSwing Condenser NA 0.9/ 0.25
FocusingCoaxial Coarse and Fine Adjustment, Fine Division 0.001mm, Coarse Stroke 37.7mm per Rotation, Fine Stroke 0.1mm per Rotation, Moving Range 24mm
StageDouble Layers Mechanical Stage 185×142mm, Moving Range 75×55mm
Photo AdapterUsed to connect Nikon or Canon DLSR camera to the microscope
Video Adapter1× or 0.5× C-mount adapter
Transmitted Kohler IlluminationExternal Illumination, Aspherical Collector with Kohler Illumination, Halogen Lamp 6V/30W, Brightness Adjustable
External Illumination, Aspherical Collector with Kohler Illumination, Halogen Lamp 24V/100W, Brightness Adjustable
3W LED Illumination, Brightness Adjustable
5W LED Illumination, Brightness Adjustable
Reflected Light SourceExcitationDichroic MirrorBarrier Filter
Blue ExcitationBP460~490DM500BA520
Blue Excitation(B1)BP460~495DM505BA510-550
Green ExcitationBP510~550DM570BA590
Ultraviolet ExcitationBP330~385DM400BA420
Violet ExcitationBP400~410DM455BA455
Lamp100WHBO Ultra Hi-voltage Spherical Mercury Lamp
Protection barrierBarrier to Resist the Ultraviolet Light
Power SupplierPower Supplier NFP-1, 220V/ 110VVoltage Interchangeable, Digital Display
Immersion OilFluorescent Free Oil
FilterNeutral ND25/ ND6 Filter
Centering Target

Note: ●Standard Outfit, ○Optional

Video 1: Main Body and Performance Overview:

Video 2: Imaging Capability: Brightfield imaging with transmitted or Reflected Light

Video 3. Phase Contrast Kit and Sample Images

BUM-PHK Phase Contrast Kit for Upright Biological Microscopes is composed of 10x, 20x, 40x , 60x and 100x (oil) Phase contrast objective lenses, green filter, Phase alignment tool and turret condenser with different annulus.
In this video we look at the samples images of an Onion Skin thins section slide and Tilia (basswood) thin section slide, using this kit installed on Bioimager BUM800FL upright biological microscope and connected a 2.3MP USB3.0 CMOS Colorful Camera as well as a 0.5x C-Mount Adapter.

Video 4. LED Fluorescent Attachment

This video demonstrates Bioimager BUM360FLED-A LED Fluorescence attachment to use with a standard upright microscope. It comes with 5W LED of Violet (V), Ultraviolet (UV), Blue (B) and Green (G) LEDs, and its correspondent excitation and emission filters. The video demonstrates the optics and mechanics as well as sample images at different magnifications.

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