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BIM800FLS Inverted Epi-Fluorescnce Biological Microscope


Inverted Fluorescence Microscope
10x, 20xPh, 40x lenses incldued, with tens of optional LWD lenses
Positive & Negative Phase Contrast Lenses
GFP, RFP and Optional Dapi Filters
XY stage with 96-well plate markers
100W HBO/Mercuyry illumination

$USD 4,777.00 $USD 6,000.00

Introduction

BIM800FLS Inverted Fluorescent Biological Microscope is specifically designed for the observation of cell culture with high quality optics and advanced fluorescent imagig capability. Infinity correcetd optical system gives excellent imaging performance. Super high resolution fluorescent objectives are offered to generate high quality fluorescent images. This user-friendly microscope acquisition systems allows undergraduate and graduate students with various degrees of microscopy experience to examine a wide range of samples  such as live cells, fixed cell, histological preps, microfluidic devices, bead samples, range of fluorophores, etc with high reproducibility.

Overview

  •  UCIS Infinity Independent Achromatic Optical System
  •  WF10x plan eyepieces, 20mm field of view, high eye point up to 21mm
  • Seidentopf binocular viewing head; inclined45º
  • 52-75mm interpupillary distance settings
  • ±5 Diopter adjustment
  • Quintuple nosepiece inward facing with positive click stops
  • Infinity long work distance plan Achromatic 10x, 20x, 40x and Infinity long work distance phase-contrast 20x objectives
  • Right hand controls, low position, fixed stage, 240mm x 260mm, movement range 135mm x 85mm
  • N.A. 0.30 Abbe condenser with iris diaphragm, with phase-contrast slide, long work distance 72mm.
  • Coaxial coarse and fine focus mechanism with markings. Fine focus sensitivity 0.001mm
  • 6 volt 30 watt halogen illumination
  • Fluorescence illumination system: 100W mercury lamphouse and power supply, B/G/UV/V fluorescence filter block
  • Dust cover, power cord

Applications

It can be used to observe transparent livings which are not stained, particularly living cells, tissues, fluids, sediments etc. It is an ideal instrument for biology, cytology, oncology, genetics, immunology and other research works. It can be used in scientific research institutes, universities, medical care centers, disease prevention, quality inspection, agriculture, animal husbandry and other departments. It can also be used in venereal disease examination and immune diagnosis.

Features

 Epi-Fluorescent Objective lensesInfinity flat-field fluorescence objective lens, providing high-quality optical imaging and ensuring clear images.
Negative & Positive Phase Contrast Lenses & Kit (optional)
BIM800FLW Inverted Epi-Fluorescence Biological MicroscopeBright Fluorescnet iluminatin
High-quality filters (Chroma)
Side video port Side video port

BIM800FLS Inverted Epi-Fluorescnece Biological Microscope

BIM800FLW/bim800fls.jpg
Optical System
fluorescent/BIM700FLW-nosepice.jpg

Ask us how to add or swap with Japanese objective lenses and save lots of money (1.25x, 2.5x, 4x, 10x, 20x, 40x (dry or oil), 50x (dry or oil), 60x (dry), 100x (oil)).

binocular/BIM200-07.jpgBIM800FLW/bim800-Phase-slider.JPG
Bright Field & Fluor Objective LensesPhase Contrast Objectives and Slider

Mechanical XY Stage

Attachable Mechanical Stage, X-Y Coaxial Control,Moving Rang120mm×78mm (Optional)

BIM800FLW/bim800flw-96wellplate-Green-1.JPGBIM800FLW/BIM700FLW-XY.jpg
 Ruler to show a micro-well location in a 96-well plate

 

binocular/BIM200-13.jpgbinocular/BIM200-12.jpgbinocular/BIM200-11.jpg
 35mm Culture Dish Holder 54mm Culture Dish Holder  & Glass slide holder 65mm Culture Dish Holder & Terasaki Holder

Video Imaging

BIM800FLW/bim800-side-camera.JPGBIM800FLW/bim800-side-vidoe-switch.JPGBIM800FLW/bim800-tablet-camera.JPG
Easy instal a camera

from the side video port

switch between eyepieces & camera (0/100%),

perfect for epi-fluor application

Instal a side tablet camera 

for professional observation

Epi-Fluorescent Compnents:

Filter  Sets: B, G, and optional UV, V on a 3-spot filter slider, from Chroma:

Excitation filter B/G/U/V, CHROMA Brand

  • Blue Excitation Filter, BP460~490, DM500, BA520
  • Green Excitation Filter, BP510~550, DM570, BA590
  • Ultraviolet Excitation Filter, BP330~385, DM400, BA420 (optional)
  • Violet Excitation Filter, BP400~410, DM455, BA455  (optional)
fluorescent/democubes.jpg

Ask us how to add a new filter set with any specifiction you want:

 illumination

 BIM800FLW/bim800-HBo-lamp-house.JPG SAMSUNG CSC[/caption]Epi-fluorescent Illumination Alignment Tool / Objective Lens
100W Mercury Lamp-HouseHBO Power SourceFluor alignment obj lens (optional, recommended)

Specification

Optical SystemUCIS infinity independent achromatic optical system
FrameOne-piece construction from arm to base
HeadSeidentopf trinocular head, 30° inclined, interpupilary distance 52-75mm, diopter adjustable
EyepiecePlan WF10x/20mm, plan, high eyepoint up to 21mm,
Centering AttachmentCentering telescope
ObjectiveInfinity LWD plan achromatic objective 10x
Infinity LWD plan achromatic objective 20x
Infinity LWD plan achromatic objective 40x
Infinity LWD plan phase contrast objective 20x
Focus SystemCoaxial coarse and fine focus mechanism with markings, fine focus sensitivity 1μm, coarse range 14mm
NosepieceQuintuple, reversed type with positive click stops
StageLow position, mechanical stage, size 180x150mm, moving range 80x50mm.
Drop specimen holder (φ110)
Specimen holder
Light SourceAbbe condenser N.A. 0.30 with iris diaphragm, with phase contrast slider, long work distance 72mm
6V30W Halogen lamp house
Electrical part for 6V30W halogen lamp
6V30W Halogen bulb (Osram)
250V/3.15A Φ5×20 fuse
Power supply 110-240V 50/60Hz
Blue filter (Φ34)
Green filter (Φ34)
Yellow filter (Φ34)
Fluorescence100W mercury lamp house
100W Power supply unit
100W mercury bulb
Fluorescence illuminator
Excitation filter B
Excitation filter G
250V/5A Φ5X20 Fuse
Phase ContrastPhase contrast slider (for 10x/20x/40x phase contrast and brightfield observation)
OtherAllen screw driver 2mm
Dust cover

 

Optional AccessoriesItem No.
HeadSeidentopf binocular head, 45° inclined, interpupillary distance 48-75mm, diopter adjustableBIM800FLW-BH
EyepieceWF10x/22mm plan eyepiece, high eyepointBIM800FLW-10×22
WF10x/20mm, plan, high eyepoint up to 21mm, diopter adjustableBIM800FLW-10×20
WF16x/13mm, planBIM800FLW-16×13
ObjectiveInfinity plan achromatic objective 4xBIM800FLW-4xbf
Infinity LWD plan achromatic objective 60xBIM800FLW-60xLWD
Infinity LWD plan phase-contrast objective 10x (positive)BIM800FLW-10xPB
Infinity LWD plan phase-contrast objective 40x (positive)BIM800FLW-40xPB
Infinity LWD Plan Phase-Contrast Objective 10x (Negative)BIM800FLW10xNB
Infinity LWD Plan Phase-Contrast Objective 20x (Negative)BIM800FLW-20xNB
Infinity LWD Plan Phase-Contrast Objective 40x (Negative)BIM800FLW-40xNB
StageMechanical Stage, Size 350x208mm,Moving Range 50x50mmBIM800FLW-xy50
Adapter0.25x C-MountBIM800FLW-0.25XC
Digital camera adapter (need to know camera brand)BIM800FLW-DSLR-X
Bulb6V30W Halogen bulbBIM800FLW-6v30W
Specimen Holder125×85BIM800FLW-XY-125
Fluorescence Attachment100W DC Mercury lamp houseBIM800FLW-HBO
Power supply unit for 100W DC mercury lampBIM800FLW-HBO-PS
Epi-fluorescent illumination,multi-functional semi-reflectorBIM800FLW-RF
Excitation Filter B, CHROMA BrandBIM800FLW-IB
Excitation Filter G, CHROMA BrandBIM800FLW-IG
Excitation Filter U, CHROMA BrandBIM800FLW-IUV
Excitation Filter V, CHROMA BrandBIM800FLW-IV
100W Mercury bulbBIM800FLW-HBO-C
100W Mercury bulb (Osram)BIM800FLW-HBO-O
Empty Filter CubeBIM800FLW-F

Plenty of Accessories
BIM800FLW/bim800flw-accessories.JPG

Please visit either of the following links to select a camera for image acquistion based on your budget:

Cooled CCD Camera

sCMOS cameras

– add a Retina Display

Sample Images

BIM800FLW/cartilage_10xbf.jpg

10x Ph-postive lens, BF mode, 0.5x c-mount adapter

BIM800FLW/cartilage_20xph.jpg

20x Ph-positive lens, BF mode, 0.5x c-mount adapter

BIM800FLW/cartilage_40xpo-bf-1x.jpg
40x Ph-positive lens, BF mode, 1x c-mount adapter
BIM800FLW/cartilage_60xbf-1x.jpg
60x Ph-positive lens, BF mode, 1x c-mount adapter
BIM800FLW/cartilage_10xbf.jpg

10x Ph-positive lens, Ph mode, 0.5x c-mount adapter

BIM800FLW/cartilage_20xph.jpg

20x Ph-positive lens, Ph mode, 0.5x c-mount adapter

BIM800FLW/cartilage_40xph-pos-1x.jpg

40x Ph-positive lens, Ph mode, 1x c-mount adapter

BIM800FLW/cartilage_40xph-neg-1x.jpg

40x Ph-negative lens, Ph mode, 1x c-mount adapter

Following images were collected using this microscope, BIC-C30 3MP sCMOS Camera, BIM800-0.5x 0.5x C-mount adapter and the below-mentioned objective lenses. merged images were generated using Image software.

FluoCells® Prepared Slide #2: bovine pulmonary artery endothelial cells (BPAE) cells with Mouse Anti-α-tubulin, BODIPY FL Goat Anti-Mouse IgG, Texas Red-X Phalloidin, and DAPI), (Molecular Probes)

Obj LensUV ExcitationBlue ExcitationGreen ExcitationMerged / Superimposed 2-3 Channels
20x

BIHR-20xBF

40x

BIMJ834-40x

BIM800FLW/slide2-40x-bfp.jpgBIM800FLW/slide2-40x-gfp.jpgBIM800FLW/slide2-40x-rfp.jpgBIM800FLW/slide2-40x-rg.jpg
50x LWD

BIHR-50xBFL

 BIM800FLW/slide2-50xlwd-bfp.jpgBIM800FLW/slide2-50xlwd-gfp.jpgBIM800FLW/slide2-50xlwd-rfp3.jpg
60x

BIMJ969-60x

BIM800FLW/slide2-60x-bfp.jpgBIM800FLW/slide2-60x-gfp.jpgBIM800FLW/slide2-60x-rfp.jpg

FluoCells® Prepared Slide #6 (Muntjac cells with Mouse Anti-OxPhos Complex V Inhibitor Protein, Alexa Fluor 555 Goat Anti-Mouse IgG, Alexa Fluor 488 Phalloidin, and TO-PRO-3)
(Molecular Probes)

BIM800FLW/slide6-05x-rg.jpg

5x BIHR-5xBF

BIM800FLW/slide6-40x-rg.jpg

40x BIMJ834-40x

60x BIMJ969-60x

 BIM800FLW/slide6-20x-rfp.jpg

20x BIHR-20xBF

BIM800FLW/slide6-20x-gfp.jpg

20x BIHR-20xBF

BIM800FLW/slide6-40x-rfp-cp.jpg

40x BIMJ834-40x

fluorescent/BK-F2_G.JPG fluorescent/BK-F2_B.JPG

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