Plan Fluor Objective Lenses
BIM720FL Inverted Epi-fluorescence Microscope
30106 Confocal Glass-Bottom 6-Well Plate
$USD 374.00
Plan Fluor Objective lenses
$USD 399.00
Plan Apochromatic lenses 10x, 20x, 40x, 100x
Plan Fluor Objective lenses
$USD 399.00
Abbreviations:
BF: Brightfield,
BD: brightfield-darkfield,
DF: Darkfield,
DOF: Depth of Field (mm or um)
F: Focal Length (mm)
FL: Fluorescent, Fluorescence
FOV: Field of View (mm)
NA: Numerical Aperture
Pol: Polarized, Polarizing
RMS: Royal Manufacturing Standard, RMS Thread = 0.800″-36 or 4/5″-36
WD: Working Distance (mm)
Epi-Fluorescent Objective lenses
Apochromat lenses are the highest quality and have been corrected to bring 3 wavelengths (red, green & blue) into focus on the same plane in epi-fluorescent microscopes such as IncuScope. Apochromat lenses have a flat field of view, meaning that the curvature of the lens has been corrected so that the entire circular image is crisp and in focus.
Plan Achromat Lenses are the mid-range quality lens and have been corrected to bring 2 wavelengths (red & blue) into focus on the same plane. Plan lenses also have a flat field of view.
Achromat lenses are the basic quality lens and have been corrected to bring 2 wavelengths (red & blue) into focus on the same plane. However, without the “plan” indication – these lenses have not been corrected for the curvature of the lens and do not have a completely flat field of view – meaning the very outer edges of the circular image viewed will be slightly out of focus due to that curvature of the lens.
| Lens Type | Quality | Corrected for | Field of View |
| Apochromat | Best | 3 Wavelengths | Flat |
| Plan Achromat | Middle | 2 Wavelengths | Flat |
| Achromat | Lowest | 2 Wavelengths | Not Flat |
High NA Plan Fluor Lenses
| Magnification | NA | WD
(mm) |
F’ (mm) | R (μ) |
DF (μ) |
FOV
On object (mm) |
FOV
on image (mm) |
Thread |
| 10x | 0.40 | 3.6 | 20.0 | 0.74 | 1.65 | 2.2 | 22.0 | RMS |
| 20x | 0.75 | 0.6 | 10.0 | 0.45 | 0.59 | 1.1 | 22.0 | RMS |
| 40x | 0.95 | 0.17 | 5.0 | 0.35 | 0.37 | 0.55 | 22.0 | RMS |
| 100x (water) | 1.20 | 0.16 | 2.0 | 0.28 | 0.23 | 0.22 | 22.0 | RMS |
| 100x (Oil) | 1.35 | 0.08 | 2.0 | 0.25 | 0.18 | 0.22 | 22.0 | RMS |
RMS = 0.800″-36
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