BioStitch-500 Live Stitching/Mosaicing & Digital Slide Scanning Software with Free Camera
This is the most economical solution to convert your microscope to a digital slide scanner. You can scan a slide manually with a manual XY stage, and stitch all images at any magnification you like to use. You can use this with an upright, inverted or stereo microscope and simply by combing this camera with a C-mount adapter and the software we offer.
The software also supports live extended depth of field (EDF) feature.
What do you need?
By using your own microscope equipment, you only need to buy this software-camera package then to be able to do whole slide imaging within a minute. This’s just a fraction of a slide scanners cost!
To have a digital slide scanner, with minimal cost and lots of options, you need these items:
1- A microscope
You can use an inverted, upright or stereoscope, depending on the application. Upright biological microscopes are very common for slide scanning, due to the easy handling of slides and also the availability of automated slide loader. Inverted microscopes will be an advantage if you cannot flip over your sample if you have a sample without coverslip. Stereoscopes are commonly used for a large sample of minerals and polarized samples.
2- Manual XY stage
You need a good mechanical XY stage to move your sample in X and Y directions / horizontal and vertical directions to scan the big region of your sample. If you need this or a glass slide holder, just contact us or select from the optional items.
Note: You can use a high-precision XY stage for magnifications over 40x.
3- A C-Mount adapter
To connect the camera with your microscope. Using the proper size of the c-mount adapter is matter. For a 1/2″ sensor, you will need a 0.5x C-mount adapter. It would be better you use a 0.66x or even 1x c-mount adapter to have the maximum flatness of your image. If you do not have a plan apo obj lens or you do not like to spend money on these expensive lenses, using a higher magnification of c-mount adapter is a very good solution.
4- A Camera and Software
We offer several models of cameras.
|Sensor||1/1.8″||2/3”||1″(14.13×7.45)||1″(13.06mm x 8.76mm)|
|Pixel Size||3.45×3.45um||3.45 x 3.45um||3.45×3.45||2.40μm x 2.40μm|
|Resolution||2048 x 1536|
|2448 x 2048|
|35 fps (1×1)|
88 fps (2×2)
For example, this scan (link below) is 72,079 x 35,892 pixels, i.e. 2,587 MegaPixels or 2.6 GigaPixels with ~500 Megabites file size (compressed). Of course, this takes some time to scan an area like that manually but it is possible.https://www.easyzoom.com/image/92839/popular
Example Video using BioStitch-300 for Live Stitching
Some Hints:1. Using lowest exposure time such as 50ms or so is always suggested.2. Using a higher magnification of c-mount adapter helps to generate a flat and uniform image.3. Start with low magnification for initial practice and figuring out the large map of the sample then use higher magnification for some ROIs.4. As you know well, when you switch from 10x to 20x obj lens or 20x to 40x, you magnify the image and increase resolution double, BUT you get the field of view (FOV) four times smaller (twice in X- and twice in Y-direction.). This means you need to spend 4x longer time to cover the same FOV at higher magnification.5. If you have a tilted sample, when you move the sample with XY stage, use your other finger to adjust the focus gently.Extended Depth of Field (EDF):