IncuScope IS500 Brightfield / Fluorescence Inverted Incubator Microscopes

IncuScope IS500 Incubator Microscope:  Small, Smart & Simple

What is an IncuScope?
The IncuScope is a low price, small, compact, USB-based, portable digital inverted brightfield and epi-fluorescence microscope. The microscope fits on a shelf inside an incubator or inside a hood. It has a built-in digital camera and light source. This runs on a laptop or desktop using just one USB cable and captures fluorescence and/or brightfield images. Available with a variety of objective lenses from 1.25x to 100x. The software allows doing time-lapse study and controlling the light to turn on/off to avoid photo-bleaching and photo-toxicity. Thus, this saves paying for heating stage or miniature incubator, mechanical shutter, digital camera and fluorescence attachment which are important components for live cell imaging application. Its price is the lowest in the live cell imaging field. A side-by-side comparison with similar products such as Lumascope, Juli, EVOS, this has better images. When it comes to the price, this is the most economic choice. Last but not least, you can check your cell culture inside an incubator via Internet or smart-phone if you connect the IncuScope computer to Internet / WiFi and use our remote-control software.

Contact Us for Free Demo, a Quote , and Custom Design with 6 colors or OEM.

Why IncuScope?

There is no greatness where there is not simplicity Leo Tolstoy, quoted in The week.

• Simple: the most simple digital microscope in the world, requires no setup, almost no training, instant installation, familiar PC environment.
• Affordable: The lowest price video time-lapse microscope in the world.
• Your cells are always happy since they never leave incubator.
• Using our remote-control software/app you can see inside your incubator via Internet or iPhone, Android, Blackberry

Highlighted Applications:

• Long-term live cell imaging in single and time lapse modes
• Cell doubling and movement measurements
• Stem cell differentiation movies
• More sophisticated uses are also easily achieved with the IncuScope: cell counting or hemocytometry and growth monitoring. Using the IncuScope, it is possible to monitor cells remotely through the web from within a tissue culture incubator, overnight, away from the lab.

Unlimited Scientific and Routine Applications 

• Microfluidics field, lab-on-chip, MEMS
• Viral infections, HIV/AIDS study and all level III safety labs
• Embryology, In Vitro Fertilization (IVF) studies
• Bacteriology
• Stem Cell Colony formation  study (long term live cell imaging)
• Transfections, knock-out studies such as siRNA tagged
• Drug discovery research and clinical labs
• Field microscopy, such as marine biology
• Proliferation assay
• Kinetic cell migration assay
• Kinetic angiogenesis
• Cell invasion
• Cytotoxicity
• Cell culture quality control
• Apoptosis

Colorful brigtfield images (available with iS500BF model only)

• Yields a magnification on familiar laptop displays of approximately 20× with a field of view of 1 mm square.
• Standard filter sets
• Brightfield capability included. Phase Contrast is optional.
• Time-lapse study with turn on/off light (without using a shutter!)
• USB 3.0 interface, enabling easy integration with larger systems and remote monitoring
• Small enough to fit on a desk, lab bench, in an incubator or tissue culture hood (approximately 190 x 125 x 115 mm or 7.5 x 5 x 4.5 in).
• #1 Performance/Price in the Market; it saves paying to heating stage, shutter and camera, e.g. compare it with Zeiss

Price: The best price in the imaging market:

• LumaScope LS500 ( > $8,000)
• Juli (> $15,000)
• EVOS (> $27,000)
• IncuCyte (> $77,000)

	IncuScope-500
Optics	Infinity-corrected objective with an RMS thread type
Image Resolution	1920×1200 pixels  =  2.3 MP; 1/1.9″(7.20×4.5) CMOS Sensor (3.75×3.75 microns/pixels)
Image Formats	JPEG, TIF, BMP, GIF, PNG.
Optics	Chroma or Omega Optical Filters
Image Size	100 kB-4MB
Objectives	1.25x, 2x, 4x, 5x, 10x, 20x, 40x, 50x (oil), 60x (dry), 100x (water, or air/oil) (optional)
Fluorescence (Default)	Excitation: 482/35 BrightLine Bandpass Emission: 536/40 BrightLine Bandpass Dichroic Mirror: 506 nm BrightLine Dichroic Beam Splitter
Fluorophores	GFP (EGFP), FITC (Fluorescein), Alexa Fluor488, SuperGlo GFP, wild type GFP (non-UV excitation), red-shifted GFP (S65T), BODIPY, Cy2, Calcein, MitoTracker Green, Oregon Green.
PC communication	USB 3.0 port
Power	DC adapter
LED Life Time	> 20,000 hours
Operating System	Microsoft Windows 7, 8, 10
Operating Condition	0°C- 42°C, 5%-99% RH non-condensing
Warranty	1 year

Field of Views

Field of View

1.25x: 19x19mm 2x: 9.31x7mm 4x: 7.8×5.85mm 5x: 6.8×5.11mm 10x: 3.4×2.55mm 20x: 1.52×1.14mm 40x: 7.8×5.85mm

Microscope Models:

Model	Description
iS510	Brightfield and Epifluor Imaging (Per specification table) includes iS500-OEM plus focus, enclosure no obj lens, no XY stage
iS500BF	iS500 with Brightfield imaging Only
iS510-OEM	Brightfield and Epifluor Imaging includes just the epi-fluorescnce module (filter cube, filters), BF and FL LEDs, camera, PCB and software
iS510T	iS500 with Tablet PC or Retina Display
iS520	iS500 with BF and 2 Fl channels
iS530	iS500 with BF and 3 FL channels

IncuScope Accessories

• Customized any fluorescence filters from UV to Infrared
• Various Camera Selections, i.e. different resolution (1.4MP to 16MP), speed (1-90 fps) and chip size (1/3”-1”).
• Objective lenses: 1.25x, 2x, 4x, 5x, 0x, 20x, 40x, 50x (oil), 60x (dry), 100x (water, or air/oil)

1. LED Selection

You select the LEDs, we will select the proper excitation emission and barrier filters.

LED	Nominal Wavelength	Minimum LED Power Output	Applications/ Fluorophores
Deep UV	265 nm	10 mW
Deep UV	280 nm	25 mW
Deep UV	300 nm	40 mW
Deep UV	340 nm	53 mW
UV	365 nm	190 mW
UV	365 nm	1150 mW
UV	375 nm	387 mW	Dapi/Hoecst
UV	385 nm	270 mW
UV	385 nm	1650 mW
UV	395 nm	400 mW
UV	405 nm	870 mW
UV	405 nm	1500 mW
Violet	420 nm	750 mW
Royal Blue	450 nm	1850 mW
Royal Blue	455 nm	900 mW
Blue	470 nm	650 mW	GFP
Blue	490 nm	255 mW
Cyan	505 nm	400 mW
Green	530 nm	350 mW
Lime	565 nm	880 mW
Amber	590 nm	160 mW	RFP/ Texas Red
Amber	595 nm	445 mW
Orange	617 nm	600 mW
Red	625 nm	700 mW
Deep Red	660 nm	940 mW
Far Red	730 nm	515 mW
IR	780 nm	200 mW
IR	780 nm	800 mW
IR	810 nm	325 mW
IR	850 nm	900 mW
IR	850 nm	1400 mW
IR	880 nm	300 mW
IR	940 nm	800 mW
IR	970 nm	35 mW
IR	1050 nm	50 mW
IR	1200 nm	30 mW
IR	1300 nm	25 mW
IR	1450 nm	31 mW
IR	1550 nm	31 mW
Broadband	470 – 850 nmf	70 mW
Warm White	3000 Kg	570 mW	Brightfield
Warm White	3000 Kg	2000 mW
Cold White	6500 Kg	800 mW
Cold White	6500 Kg	2350 mW

2. Cameras

Order Code	Sensor & Size (mm)	Pixel (μm)	G Sensitivity Dark Signal	FPS/Resolution	Binning	Exposure
BIC-T3C-12C	12MP / IMX226(C) 1/1.7″ (7.40×5.55)	1.85×1.85	280mv with 1/30s 0.1mv with 1/30s	Q3/coming soon	1×1,2×2	0.1ms~2000ms
BIC-T3C-6C	6.3MP / IMX178(C) 1/1.8″ (7.37×4.92)	2.4×2.4	425mv with 1/30s 0.15mv with 1/30s	[email protected]×2048 [email protected]×1024	1×1,2×2	0.244ms~2000ms
BIC-T3C-3C2	3.1MP/IMX123(C) 1/2.8″(6.46)	2.5×2.5	600mv with 1/30s 0.15mv with 1/30s	[email protected]×1536 [email protected]×768	1×1,2×2	0.244ms~2000ms
BIC-T3C-2C1 (Default)	2.3M / IMX185(C) 1/1.9″(7.20×4.5)	3.75×3.75	1120mv with 1/30s 0.5mv with 1/30s	[email protected]×1200	1×1,2×2	0.244ms~2000ms
BRC-1600	16MP / MN3412 (Color) 1/2.33″	1.335 x 1.335	1200mV	4fps @4608×3456 16 @ 2304 x1728	1×1,2×2	1ms-999ms

We also offer CCD and sCMOS cameras, either USB 3.0 or USB2.0. Please contact us for further assistance.

3. Obj lenses

Types:

a) plan achromat

b) plan semi-apo

c) Plan apo

Magnifications: 1.25x, 2x, 4x, 5x, 10x, 20x, 40x, 50x, 60x and 100x (oil, dry or water)

4. Accessories

Mechanical stage can be installed from both right and left sides easily, even from the front.	The mechanical XY stage grabs a micro-well plate directly without having any holder)
all customized shape of sample holders	T-25 Flask holder T-75 Flask holder are also available.
dimensions for the Terasaki holder. 35-mm petri-dish holders needs this adapter.

incuView Software Interface

Features:

Time-lapse mode

Controls each LED separtely

Turn ON/OFF option for teh LEDs, i.e. digital shutter

Live view and image capture

label image color

Merge images

Color and monochrome images

Manual and automatic exposure time and gain

Captures BF and FL images separately

Zoom in / Zoom out with a touch of mouse wheel

Full screen of the live image, resizable tabs

1.25x Obj Lens	2x Obj Lens
4x	5x
10x	20x
40x with 16MP camera	50x obj lens with 2.3MP camera
100x (Water)

Colorful brigtfield images (available with iS500BF model only)

BIM5-4x 4X Plan Achromat, Cartilage, 16MP Camera	4x Plan Semi Apo, Cartilage, 16MP Camera
BUM5-10x 10x Plan Semi Apo, Cartilage, 16MP Camera	20x Plan Apo, Cartilage, 16MP Camera
BIM5-40x 40x Plan Obj lens, Cartilage, 16MP Camera	50x Plan Semi Apo Obj lens, Cartilage, 16MP Camera

Following images were collected using 3 LEDs of UV, Blue and Green excitations, 3MP sCMOS camera BIC-C30, and the below-mentioned objective lenses. merged images were generated using IncuView software.

FluoCells® Prepared Slide #2: bovine pulmonary artery endothelial cells (BPAE) cells with Mouse Anti-α-tubulin, BODIPY FL Goat Anti-Mouse IgG, Texas Red-X Phalloidin, and DAPI), (Molecular Probes)

BIHR-20xBF PlanFluor EPI20XBF Plan Semi Apochromat 20X/NA0.45/WD3 Lens:

BIMJ969-60x: 60x /NA0.80/WD0.23 Plan Objective Lens:

FluoCells® Prepared Slide #6 (Muntjac cells with Mouse Anti-OxPhos Complex V Inhibitor Protein, Alexa Fluor 555 Goat Anti-Mouse IgG, Alexa Fluor 488 Phalloidin, and TO-PRO-3) (Molecular Probes)

BIMJ969-60x: 60x /NA0.80/WD0.23 Plan Objective Lens: