Introduction BIM1000A inverted research microscope
BIM1000A was creatively designed front panel as a touch screen, which makes human-computer interaction more convenient, powerful and expandable. Left and right sides reserved knobs and buttons, even in a dark lab, it is easy to control, which allows researchers to focus more on the experiment itself, rather than complex microscope operation.
BIM1000A Advanced Inverted Microscope for Research
BIM1000A provides an advanced imaging platform with 25mm field of view, to meet experimental needs of large flux observation. With high speed full frame CMOS camera, which has greatly improved shooting efficiency. Full-motorized BIM1000A-AT and coded BIM1000A-M realize integration of multimodal imaging technology such as bright field,fluorescence, DIC Nomarski, phase contrast and so on, both models can choose single or double layer optical path,anti-focus shift module can be optional which is more suitable for long-time dynamic imaging of living cells. BIM1000A provides stable and reliable platform for confocal, super resolution,single molecule and other advanced microscopic imaging technology.
In addition, the touch screen could achieve smooth control of electric components while recording status of each sensor, recording user usage habits, switch application scene by one-click. Combined with powerful acquisition and analysis software, BIM1000A is full of stability and scalability, meet a variety of experimental needs,provide reliable, repeatable data.
BIM1000A Advanced Inverted Research Microscope Features
Large field illumination module:
Bright and large area transmission illumination
Transmission illumination module adopts high power LED illumination to guarantee full field of view brightness and uniform illumination, ensure get clear, consistent results from high magnification DIC applications, provide stable light source for seamless image stitching.
Large aperture reflective fluorescent illuminator
A compact optical fiber illumination device for FOV25mm fluorescence imaging is designed, equipped with high power LED light box, provides wide spectrum high transmittance lighting including ultraviolet light, meanwhile compatible with large aperture fluorescent filters, provide fluorescence images of FOV25mm with high signal-to-noise ratio
Large aperture observation optical system:
Equipped with large diameter tube lens to expand light
flow,cooperate with large target surface CMOS sensor, both
bright field and fluorescence imaging could FOV25mm field of
view.
FOV25 imaging objective:
The objective with superior image flatness guarantees high
quality images. Maximize potential of FOV25 objectives
significantly speeds up data collection.
Camera for large volume data acquisition:
NEXCAM-MAX2400 High sensitivity monochrome camera, full
frame sensitive Chip, target surface 36.0×23.9mm, 24 million
pixel CMOS Image sensor,maximum acquisition speed could
reach 114fps, realize digital imaging of the largest field of
vision.
Living cell culture system:
Living cell culture system is designed for
imaging observation of live cells. Precisely
control temperature of microscope platform
while maintaining the internal temperature,
humidity and CO2 concentration of culture dish,
providing stable cultivation environment. for
long-term live cell experiments.
AFS realize stable and reliable imaging results
Focusing stability is key factor of live cell imaging, even in constant temperature and humidity environment, focus position of microscope also varies slightly because of temperature and vibration changes, which greatly affects long-term observation of living cells.bim1000A adopt independent focusing design, maximumly reduce impact on Z axis from other mechanical components. Brand new designed APS (Adaptive Focus System), adaptive anti-focus shift system, eliminate focus shift. Even when using high-magnification, highnumerical aperture objectives and advanced imaging technologies, such as super-resolution, confocal, TIRF, can still be clearly focused.
High specification LED fluorescent illumination system
LED 4 enables up to 4 channels LED illumination, highly matching
fluorescent dye commonly used in market, excitation energy concentrated,
high brightness, meet the daily experimental fluorescence imaging need.
long life, do not need to change bulb. Compared to traditional mercury
HBO lamps,it reduce photobleaching and phototoxicity,which are very
friendly to live cell samples.It is an ideal microscope light source which is
sustainable, energy saving and low carbon, environmental protection.
Front 5.6 inch touch screen
Touch screen control movement of electric parts such as objective, double
layer/single layer fluorescent turnable, condenser,light intensity, motorized
platform speed, motorized Z-axis speed, main body splitting port, ESC
escape,FN key, objective focus. Display real-time, display objective
magnification, transmitted illumination brightness, fluorescence band,
output port, XYZ position and speed and other component status.
High speed electric control
Operation and conversion speed of objectives,filters, the XY stage and the observation module are reatly improved, enabling an easy operating environment for researchers to concentrate on routine observations and images. The joystick that can control the stage freely allows the microscope working as your eyes and hands, making it easy to use.
Joystick with built-in touch panel
The joystick comes with touch screen, which can set the state of main body, motorized platform control by multi-level and also motorized focus, built-in function key and AFS focus stabilization control.
Unparalleled optical system
BIM1000A high quality infinity optical system, equipped with bright field, fluorescence, phase contrast, DIC, hoffmann and other complex observation methods, no matter which observation method, you can get bright high signal-to-noise ratio of the original image, with its excellent optical performance and reliable reliability are highly valued by researchers.
Phase contrast
The condender has built-in phase contrast ring, which can be converted from bright field observation to phase contrast observation by turning the condenser turntable. Semiapochromatic phase contrast objective provides clear imaging with high contrast.
Fluorescence
Using the newly designed filter group, cutoff depth of singlechannel fluorescence filter reaches OD6, and the image with high signal-to-noise ratio can be obtained, which can be used for single-molecule and single-cell experiments.illumination module spectrum design covers a wide wavelength range,
and six-hole filter module turntable meets needs of multi-color fluorescence. High power LED fiber optic light source is bright and safe.
DIC(Differential interference)
High quality large field of view differential interference optics
system covers all magnifications, providing uniformly clear
and detailed images with high resolution and contrast for each
sample.
Hoffmann contrast
High contrast ratio phase contrast imaging technique for
unstained, transparent samples such as egg cells. Hoffmann
phase contrast components provide pseudo-three-dimensional
image with the appearance of shadow casting. The contrast
direction of each sample can be easily adjusted.
Electric control (grating type) : travel range 130 mm x 100 mm (table 445 mm x 300 mm), Maximum speed:20mms; Resolution: 0.1um; Repeatability: ±0.2μm, can be equipped with multi-well plate, 35mm petri dish and slice three special sample clamp adapter; Well Clamper is optional;
Manual stage
Three-layer mechanical moving stage, stage size :340X230mm, moving range 130×85mm
Focusing system
Electric Z-axis, travel up 8.5mm down 1.5mm, resolution 0.01μm, repetition accuracy ±0.02μm (10nm grating); 3-speed focusing handwheel (2μm/ turn, 40μm/ turn, 200μm/ turn);
Touch LCD
in front of main body, display control light source intensity, objective magnification, fluorescence band, intermediate magnification,turnable position, bertrand lens etc.;
Intermediate magnification switching
Manual 1 x, 1.5 x switching
Bertrand lens
Manual switch in and out of light path, focus adjustable
Port
Electric switching splitting ratio: left side: visual =100:0; Right side: Visual =100:0; Bottom: Visual =100:0;
Illumination system
Transmitted kohler illumination, 3W LED illumination; Field of view/aperture diaphragm; bend arm can tilt 25°; Electric optical brake optional.
EPI illumination: LED optical fiber (wavelength:385nm,470nm,555nm,630nm); Field of view/aperture diaphragm; 2 hole filter insert plate; Lamp source controller; Electric brake (software control) optional
6-hole electric fluorescent turntable (B, G, U, R standard); φ25/30 light pass; Electric optical brake; 6-hole manual fluorescent turntable (B, G, U, R standard); φ25/30 light pass; Manual optical brake;
DIC prism insert can be placed in nosepiece slot; DIC, polarizer, analyzer slide plate;
Controller
XYZ electric control; Display objective magnification, fluorescence band and other states; Shortcut key setting;
Software
NOMIS advance 5.0 image analysis software
Host side port
1× relay lens Confocal left port; ·1.8x M52 camera relay lens
System Diagram
Nomis Pro X microscope control and analysis
Nomis Pro X, software platform of BIM1000A , integrates hardware control, mage acquisition and processing into one software. Nomis Pro X makes it easier for you to use this microscope, even for various complex microscope operations.It can also be quickly realized through the linkage of hardware and software. Image processing includes image measurement, image adjustment, filtering, noise reduction, deconvolution, SIM reconstruction, etc., which can meet the needs of different work applications.
Multi-dimensional imaging
Able to remember customized viewing modes for multiple imaging needs such as multi-channel, multi-position, time-lapse imaging or large image stitching.
Multi-dimensional image display
Multi-channel situation, delay, Z-axis stacking, XY axis position and image stitching information of the image can be visually displayed in one window to improve the repeatability of experimental results
High speed hardware control
Various electric components of the microscope controlled by software, such as objective conversion, focusing, condenser conversion, fluorescence module conversion, etc.
Deconvolution
The two dimensional image can be deblurred. It can be deconvolved multiple times, and results can be viewed and adjusted on screen. 3D deconvolution can also be used for
multi-dimensional images.
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