Multi-wavelength microscopy LED light sources employ the state-of-the-art dichroic mirrors to combine multiple LEDs into one single beam with high optical efficiency, without the need of using any mechanical moving parts. As a result, customers can turn On/Off or modulate any LED or any combination of LEDs at ~μs acuracy, and the output power of each and every LED can be adjusted individually or in unison and with high precision.
Bioimager offers high-power microscopy LED light sources to produce highly spectral-selective, high-intensity, homogeneous wide-field illumination delivered to sample via objective lens. Combine Bioimager high-power LED light sources with one of the BioimagerLED controllers to take advantage of the fast-switching and quick time-response of LED technology.
Bioimager’s multi-wavelength microscopy LED light sources employ the state-of-the-art dichroic mirrors to combine multiple LEDs into one single beam with high optical efficiency, without the need of using any mechanical moving parts. As a result, customers can turn On/Off or modulate any LED or any combination of LEDs at ~μs acuracy, and the output power of each and every LED can be adjusted individually or in unison and with high precision.
Multi-wavelength Reflective Microscopy LED Sources
Bioimager multi-wavelength Microscopy LED Solution is designed with flexibility and upgradability in mind: the light sources are constructed using ‘standard’ building blocks, and hence they are scalable and reconfigurable. Customers can easily add new wavelength and/or change/remove wavelength in his setup with most reusability of the parts that they have purchased.
As illustrated below, the basic setup of this multi-wavelength microscopy LED solution consists of:
A Multi-wavelength Microscopy LED Systems;
LED control module(s);
A microscope adapter.
Note: Up to eight (8) wavelengths can be configured into one system.
2. Number of Channels
3. Wavelngth Portfolio
4. LED Control Modules
Simple one-stop software GUI
External TTL triggered operation
20µs minimum pulse width
Up to 25kHz modulation frequency
Compatible with cameras with Strobe-out feature
Ideal for fast switching between different LED wavelengths, and for working with electrophysiology rig through TTL trigger signal. This option also works well to replace the short-life mechanical shutter, and synchronize short LED pulses with camera exposure. This reduces photo-bleaching of the sample under constant illumination.
Simple but highly-precise potentiometer-style adjustment of constant intensity
Alternative analog voltage-control
Arbitrary waveform by analog voltage modulation
Modulation frequency of up to 100kHz using external analog control
The manual/analog voltage option allows researchers to take full potential of solid-state LED’s speed to meet the need of their application. This is the option for users who need to modulate the LED intensity with arbitrary waveforms other than square wave. The manual control mode is ideal for users who prefer the convenience of a PC-less control of LED intensity. Switches on the front panel are used to easily select between manual and analog voltage-control.
5. Microscope Adapters
Microscope Make & Model
Leica DMI microscope
Nikon Eclipse microscope
Nikon LV-UEPI/2/A microscope
Olympus IX & BX microscope (multi-wavelength)
Zeiss Axioskop microscope
*If you don’t see your microscope make & model, please contact us.
one (1) set of necessary control module(s) to achieve the control option you prefer,
one (1) microscope adapter. Below are some popular 2-LED combinations.
Other combinations of your choice are possible. Default multi-wavelength beam-combiner(s) typically used are listed at the check out, but they can be swapped with alternative of your choice.
The control modules are modularized fully-customizable turn-key solutions for optogenetics, fluorescence excitation, and other biophotonics applications. Precisely-timed and high-intensity light pulses are required in optogenetics experiments to activate channel-rhodopsins (ChR2, ChR1 etc.) and halorhodopsins (NpHR) in order to excite and inhibit neurons. To meet these requirements, we have developed a proprietary “IntelliPulsing” technology to allow BLS-series sources to output significantly higher power in pulse mode than what the LEDs are rated for in CW mode.
The control module features a linear LED driver design that eliminates light intensity ripples and oscillations often observed when low-cost buckpuck nonlinear drivers are used. Clean and highly repeatable pulses are critical to quantitative experiments. Both CW mode and pulse modes are supported. Time resolution of the control module is 20us and light intensity can be adjusted with 0.1% increments. Each driving channel on the control module has its own TTL trigger input. Rising edge, falling edge, and follower mode are supported in the trigger mode.
This light source solution comes with a Windows-based operation software featuring an intuitive yet powerful graphic user interface. A software development kit (SDK) is also provided for user integration into environment such as Labview and Matlab.
An optional touch-screen control pad is available (see photo below), with which customers can control LEDs without a computer.
The control module can also be operated without being connected to a computer or a touch-screen pad. This is because custom-defined pulse sequences can be programmed and stored into the control module (through software), after which the light sources can be operated alone and triggered by external TTL pulses.
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