English/Metric Calibration Slide /Stage Micrometer

$USD 99.00


Stage Micrometer, Calibration Slide with both Metric & English measurements.
1″ / 25mm length with 0.1mm divisions.
Perfect for every microscopes, particularly for stereoscopes.

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Introduction to a calibration slide

A calibration slide for a microscope is a specialized slide that is used to calibrate and standardize various aspects of microscope imaging, such as magnification, resolution, and focus. It is a tool that helps to ensure that microscope images are accurate, consistent, and reliable.

A calibration slide typically includes a set of targets with known dimensions, patterns, and colors. These targets can be used to perform various types of calibration, such as:

  1. Magnification Calibration: By measuring the size of the targets in the calibration slide, the magnification of the microscope can be determined and adjusted.
  2. Resolution Calibration: The patterns on the calibration slide can be used to determine the resolution of the microscope, which refers to the smallest distance between two points that can be distinguished by the microscope.
  3. Focus Calibration: The targets on the calibration slide can be used to adjust the focus of the microscope, ensuring that the image is sharp and clear.

Calibration slides are available in different types, depending on the specific requirements of the microscope and imaging application. Some common types of calibration slides include:

  1. Stage micrometer: A stage micrometer is a calibration slide with a scale of precisely measured markings that can be used to calibrate the magnification of the microscope.
  2. USAF 1951 resolution test target: A USAF 1951 resolution test target is a calibration slide that includes a series of line patterns that can be used to determine the resolution of the microscope.
  3. Color calibration slide: A color calibration slide includes a set of color patches that can be used to adjust the color balance and intensity of the microscope imaging system.

Using a calibration slide can help to ensure that microscope imaging is accurate, reliable, and consistent. It is a useful tool for researchers, technicians, and others who rely on microscope imaging for their work.


Features of a calibration slide

  • Division lines: 0.02 mm (20 micron) , 0.1mm and 1mm
  • Slide materials: Optical glass
  • Total length of image: 1 mm with 100 divisions , 10 mm with 100 divisions
  • Slide size: 3 inches X 1 inch / 25mm x 75mm
  • English and metrics measurement division lines

Stage Micrometer, Calibration Slide with both Metric & English measurements

A stage micrometer or calibration slide is a glass slide with some scales on it such as millimeter, micrometer or inches. This is normally used to have a precise measurement of acquired images or add a correct scale bar after acquisition on an image for reference purpose.

BICS-0.01M-E Calibration Slide / Stage Micrometer

Microscope micrometers are commonly used for measuring or counting specimens. Eyepiece micrometers (also referred to as “reticles”) are small glass discs with markings on them. The micrometer is mounted in one of the two eyepieces and superimposes an image of the markings over the image of the specimen. Stage micrometers are slides with markings of a known dimension that, when viewed under the microscope, can be used to calibrate eyepiece micrometers. Calibration instructions are available here.


Since the size of the eyepiece micrometer image (e.g. a linear scale) appears the same even though the specimen can be magnified to a greater or lesser degree, the markings on the micrometer do not by themselves indicate any particular measurement. When a stage micrometer with known dimensions is viewed through an eyepiece micrometer, however, the measurement indicated by the eyepiece micrometer at that magnification can be noted and later used to make accurate measurements.

The micrometer slides allow you to calibrate your images based on the magnification in use. After calibartion, you will know the actual pixel size and then add a scale bar to your images.

Glass Size: 1.5” x 3” (38mm x 75mm).
Scale Length: 1 inch (English) X & Y axis
25 mm (Metric) X & Y axis
Divisions: English = 0.0005”; Metric = 10µm (0.010 mm)
With numerical labeling every 0.1 mm and 0.05”

Use any imaging software that comes with your camera, to calibrate your images.

Calibration instructions are available here.


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