Features
Infinity flat-field fluorescence objective lens, providing high-quality optical imaging and ensuring clear images. |
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Bright Fluorescnet iluminatin |
Filter wheel with 5 spots | |
Side video port |
BIM-800FLW Inverted Fluorescent Biological Microscope is specifically designed for the observation of cell culture with high quality optics and advanced fluorescent imagig capability. Infinity correcetd optical system gives excellent imaging performance. Super high resolution fluorescent objectives are offered to generate high quality fluorescent images.
Infinity flat-field fluorescence objective lens, providing high-quality optical imaging and ensuring clear images. |
|
|
Bright Fluorescnet iluminatin |
Filter wheel with 5 spots | |
Side video port |
Introduction
BIM-800FLW Inverted Fluorescent Biological Microscope is specifically designed for the observation of cell culture with high quality optics and advanced fluorescent imagig capability. Infinity correcetd optical system gives excellent imaging performance. Super high resolution fluorescent objectives are offered to generate high quality fluorescent images. This user-friendly microscope acquisition systems allows undergraduate and graduate students with various degrees of microscopy experience to examine a wide range of samples such as live cells, fixed cell, histological preps, microfluidic devices, bead samples, range of fluorophores, etc with high reproducibility.
Overview
Applications
It can be used to observe transparent livings which are not stained, particularly living cells, tissues, fluids, sediments etc. It is an ideal instrument for biology, cytology, oncology, genetics, immunology and other research works. It can be used in scientific research institutes, universities, medical care centers, disease prevention, quality inspection, agriculture, animal husbandry and other departments. It can also be used in venereal disease examination and immune diagnosis.
1. Main Body
2. Optical System
Ask us how to add or swap with Japanese objective lenses and save lots of money (1,25x, 2.5x, 4x, 10x, 20x, 40x (dry or oil), 50x(dry or oil), 60x (dry), 100x (oil)).
Bright Field & Fluor Objective Lenses | Phase Contrast Objectives and Slider |
3. Mechanical XY Stage
Attachable Mechanical Stage, X-Y Coaxial Control,Moving Rang120mm×78mm (Optional)
Ruler to show a well location in the 96-well plates |
35mm Culture Dish Holder | 54mm Culture Dish Holder & Glass slide holder | 65mm Culture Dish Holder & Terasaki Holder |
4. Video Imaging
Easy instal a camera
from the side video port |
switch between eyepieces & camera (0/100%),
perfect for epi-fluor application |
Instal a side tablet camera
for professional observation |
5. Plenty of Accessories
6. Epi-Fluorescent Compnents:
a) Filter Wheel with 5 spots
b) Filter Sets: B, G, UV, V
Excitation filter B/G/U/V, CHROMA Brand
Ask us how to add a new filter set with any specifiction you want:
c) illumination
100W Mercury Lamp-House | HBO Power Source | Fluor alignment obj lens (optional, recommended) |
GFP | RFP |
7. System Diagram
8. Specification
Specification | BIM800FLW Inverted Fluorescent Microscope | 2 | 4 |
---|---|---|---|
Optical System | UCIS infinity independent achromatic optical system | ||
Frame | One-piece construction from arm to base | ||
Head | Seidentopf binocular head, 45° inclined, interpupillary distance 52-75mm, ±5 diopter adjustment | ||
Eyepiece | WF10x/20mm, plan, high eyepoint up to 21mm, | ||
Centering Attachment | Centering telescope | ||
Objective |
Infinity LWD plan achromatic objective 10x/0.25 WD9.67 |
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Infinity LWD plan achromatic objective 20x/0.40 WD7.97, | |||
Infinity LWD plan achromatic objective 40x/0.60 WD3.76 | |||
Infinity LWD plan phase contrast objective 20x/0.40 WD7.97 | |||
Focus System | Low positioned, coaxial coarse & fine focusing system with adjustable tension control and upper limit stop, minimum reading 1μm, moving range 12mm | ||
Nosepiece | Quintuple, reversed type with positive click stops | ||
Condenser | Abbe condenserN.A. 0.30 with iris diaphragm, with phase contrast slider & filter (φ34) blue,green & yellow, long work distance 72mm | ||
Stage | Right hand controls, low position, mechanical stage, size 240x260mm, moving range 135x85mm. | ||
Drop specimen holder(φ118) | |||
Specimen holder (76×26, φ54) | |||
Light Source | 6V30W Halogen lamp house | ||
Electrical part for 6V30W halogen lmap | |||
6V30W halogen bulb (Osram) | |||
250V/3.15A Φ5X20 Fuse | |||
Power supply 110-240V,50/60Hz | |||
Fluorescence | 100W DC Mercury lamp house | ||
Power supply unit for 100W DC mercury lamp | |||
Epi-fluorescent illumination,multi-functional semi-reflector | |||
100W Mercury bulb (Osram) | |||
250V/5A Φ5X20 Fuse | |||
Excitation filter B/G, CHROMA Brand —Blue Excitation Filter, BP460~490, DM500, BA520 —Green Excitation Filter, BP510~550, DM570, BA590 |
X | ||
Excitation filter B/G/U/V, CHROMA Brand —Blue Excitation Filter, BP460~490, DM500, BA520 —Green Excitation Filter, BP510~550, DM570, BA590 –Ultraviolet Excitation Filter, BP330~385, DM400, BA420 –Violet Excitation Filter, BP400~410, DM455, BA455 |
X | ||
Phase Contrast | Phase contrast slider for 10x,20x,40x | ||
Adapter | 0.5x, C-Mount | ||
Other | Allen screw driver 2mm | ||
Allen screw driver 2.5mm | |||
Dust cover | |||
Power cord | |||
Optional Accessories | Item No. | ||
Head | Seidentopf binocular head, 45° inclined, interpupillary distance 48-75mm, diopter adjustable | BIM800FLW-BH | |
Eyepiece | WF10x/22mm plan eyepiece, high eyepoint | BIM800FLW-10×22 | |
WF10x/20mm, plan, high eyepoint up to 21mm, diopter adjustable | BIM800FLW-10×20 | ||
WF16x/13mm, plan | BIM800FLW-16×13 | ||
Objective | Infinity plan achromatic objective 4x | BIM800FLW-4xbf | |
Infinity LWD plan achromatic objective 60x | BIM800FLW-60xLWD | ||
Infinity LWD plan phase-contrast objective 10x (positive) | BIM800FLW-10xPB | ||
Infinity LWD plan phase-contrast objective 40x (positive) | BIM800FLW-40xPB | ||
Infinity LWD Plan Phase-Contrast Objective 10x (Negative) | BIM800FLW10xNB | ||
Infinity LWD Plan Phase-Contrast Objective 20x (Negative) | BIM800FLW-20xNB | ||
Infinity LWD Plan Phase-Contrast Objective 40x (Negative) | BIM800FLW-40xNB | ||
Stage | Mechanical Stage, Size 350x208mm,Moving Range 50x50mm | BIM800FLW-xy50 | |
Adapter | 0.25x C-Mount | BIM800FLW-0.25XC | |
Digital camera adapter (need to know camera brand) | BIM800FLW-DSLR-X | ||
Bulb | 6V30W Halogen bulb | BIM800FLW-6v30W | |
Specimen Holder | 125×85 | BIM800FLW-XY-125 | |
Fluorescence Attachment | 100W DC Mercury lamp house | BIM800FLW-HBO | |
Power supply unit for 100W DC mercury lamp | BIM800FLW-HBO-PS | ||
Epi-fluorescent illumination,multi-functional semi-reflector | BIM800FLW-RF | ||
Excitation Filter B, CHROMA Brand | BIM800FLW-IB | ||
Excitation Filter G, CHROMA Brand | BIM800FLW-IG | ||
Excitation Filter U, CHROMA Brand | BIM800FLW-IUV | ||
Excitation Filter V, CHROMA Brand | BIM800FLW-IV | ||
100W Mercury bulb | BIM800FLW-HBO-C | ||
100W Mercury bulb (Osram) | BIM800FLW-HBO-O | ||
Empty Filter Cube | BIM800FLW-F |
Sample Images
10x Ph-postive lens, BF mode, 0.5x c-mount adapter |
20x Ph-positive lens, BF mode, 0.5x c-mount adapter |
40x Ph-positive lens, BF mode, 1x c-mount adapter |
60x Ph-positive lens, BF mode, 1x c-mount adapter |
10x Ph-positive lens, Ph mode, 0.5x c-mount adapter |
20x Ph-positive lens, Ph mode, 0.5x c-mount adapter |
40x Ph-positive lens, Ph mode, 1x c-mount adapter |
40x Ph-negative lens, Ph mode, 1x c-mount adapter |
Following images were collected using this microscope, BIC-C30 3MP sCMOS Camera, BIM800-0.5x 0.5x C-mount adapter and the below-mentioned objective lenses. merged images were generated using Image software.
FluoCells® Prepared Slide #2: bovine pulmonary artery endothelial cells (BPAE) cells with Mouse Anti-α-tubulin, BODIPY FL Goat Anti-Mouse IgG, Texas Red-X Phalloidin, and DAPI), (Molecular Probes)
Obj Lens | UV Excitation | Blue Excitation | Green Excitation | Merged / Superimposed 2-3 Channels |
20x | ||||
40x | ||||
50x LWD | ||||
60x |
FluoCells® Prepared Slide #6 (Muntjac cells with Mouse Anti-OxPhos Complex V Inhibitor Protein, Alexa Fluor 555 Goat Anti-Mouse IgG, Alexa Fluor 488 Phalloidin, and TO-PRO-3)
(Molecular Probes)
5x BIHR-5xBF
40x BIMJ834-40x
60x BIMJ969-60x
20x BIHR-20xBF
20x BIHR-20xBF
40x BIMJ834-40x
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