BIM-800FLW Inverted Epi-Fluorescent Biological Microscope


BIM-800FLW Inverted Fluorescent Biological Microscope is specifically designed for the observation of cell culture with high quality optics and advanced fluorescent imagig capability. Infinity correcetd optical system gives excellent imaging performance. Super high resolution fluorescent objectives are offered to generate high quality fluorescent images.

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Features

Infinity flat-field fluorescence objective lens, providing high-quality optical imaging and ensuring clear images.

Infinity flat-field fluorescence objective lens, providing high-quality optical imaging and ensuring clear images.

Bright Fluorescnet iluminatin

Bright Fluorescnet iluminatin

Filter wheel with 5 spotsFilter wheel with 5 spots
Side video portSide video port

Introduction

BIM-800FLW Inverted Fluorescent Biological Microscope is specifically designed for the observation of cell culture with high quality optics and advanced fluorescent imagig capability. Infinity correcetd optical system gives excellent imaging performance. Super high resolution fluorescent objectives are offered to generate high quality fluorescent images. This user-friendly microscope acquisition systems allows undergraduate and graduate students with various degrees of microscopy experience to examine a wide range of samples  such as live cells, fixed cell, histological preps, microfluidic devices, bead samples, range of fluorophores, etc with high reproducibility.

Overview

  • UCIS Infinity Independent Achromatic Optical System
  • WF10x plan eyepieces, 20mm field of view, high eye point up to 21mm
  • Seidentopf binocular viewing head; inclined45º
  • 52-75mm interpupillary distance settings
  • ±5 Diopter adjustment
  • Quintuple nosepiece inward facing with positive click stops
  • Infinity long work distance plan Achromatic 10x, 20x, 40x and Infinity long work distance phase-contrast 20x objectives
  • Right hand controls, low position, fixed stage, 240mm x 260mm, movement range 135mm x 85mm
  • N.A. 0.30 Abbe condenser with iris diaphragm, with phase-contrast slide, long work distance 72mm.
  • Coaxial coarse and fine focus mechanism with markings. Fine focus sensitivity 0.001mm
  • 6 volt 30 watt halogen illumination
  • Fluorescence illumination system: 100W mercury lamphouse and power supply, B/G/UV/V fluorescence filter block
  • Dust cover, power cord

Applications

It can be used to observe transparent livings which are not stained, particularly living cells, tissues, fluids, sediments etc. It is an ideal instrument for biology, cytology, oncology, genetics, immunology and other research works. It can be used in scientific research institutes, universities, medical care centers, disease prevention, quality inspection, agriculture, animal husbandry and other departments. It can also be used in venereal disease examination and immune diagnosis.

1. Main Body

BIM800FLW/bim800flw-view0.JPG

BIM800FLW/bim800flw-view1.JPGBIM800FLW/bim80-top-2.JPGBIM800FLW/bim800flw-laptop.JPG

2. Optical System

fluorescent/BIM700FLW-nosepice.jpg

Ask us how to add or swap with Japanese objective lenses and save lots of money (1,25x, 2.5x, 4x, 10x, 20x, 40x (dry or oil), 50x(dry or oil), 60x (dry), 100x (oil)).

binocular/BIM200-07.jpgBIM800FLW/bim800-Phase-slider.JPG
Bright Field & Fluor Objective LensesPhase Contrast Objectives and Slider

3. Mechanical XY Stage

Attachable Mechanical Stage, X-Y Coaxial Control,Moving Rang120mm×78mm (Optional)

BIM800FLW/BIM700FLW-XY-holder.jpgBIM800FLW/bim800flw-96wellplate-Green-1.JPGBIM800FLW/BIM700FLW-XY.jpg
 Ruler to show a well location in the 96-well plates

 

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35mm Culture Dish Holder54mm Culture Dish Holder & Glass slide holder65mm Culture Dish Holder & Terasaki Holder

4. Video Imaging

BIM800FLW/bim800-side-camera.JPGBIM800FLW/bim800-side-vidoe-switch.JPGBIM800FLW/bim800-tablet-camera.JPG
Easy instal a camera

from the side video port

switch between eyepieces & camera (0/100%),

perfect for epi-fluor application

Instal a side tablet camera

for professional observation

5. Plenty of Accessories

BIM800FLW/bim800flw-accessories.JPG

6. Epi-Fluorescent Compnents:

a) Filter Wheel with 5 spots

BIM800FLW/bim800flw-WHEEL2.jpg BIM800FLW/bim800flw-WHEEL1.jpg fluorescent/BIM700FLW-wheel.jpg

b) Filter  Sets: B, G, UV, V

Excitation filter B/G/U/V, CHROMA Brand

  • Blue Excitation Filter, BP460~490, DM500, BA520
  • Green Excitation Filter, BP510~550, DM570, BA590
  • Ultraviolet Excitation Filter, BP330~385, DM400, BA420
  • Violet Excitation Filter, BP400~410, DM455, BA455  

fluorescent/democubes.jpg

Ask us how to add a new filter set with any specifiction you want:

c) illumination

BIM800FLW/bim800-HBo-lamp-house.JPG SAMSUNG CSC[/caption]Epi-fluorescent Illumination Alignment Tool / Objective Lens
100W Mercury Lamp-HouseHBO Power SourceFluor alignment obj lens (optional, recommended) 
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GFP RFP

7. System Diagram

fluorescent/BIF2702-03diag.jpg

8. Specification

SpecificationBIM800FLW   Inverted Fluorescent Microscope24
Optical SystemUCIS infinity independent achromatic optical system
FrameOne-piece construction from arm to base
HeadSeidentopf binocular head, 45° inclined, interpupillary distance 52-75mm, ±5 diopter adjustment
EyepieceWF10x/20mm, plan, high eyepoint up to 21mm,
Centering AttachmentCentering telescope
Objective

Infinity LWD plan achromatic objective 10x/0.25  WD9.67

Infinity LWD plan achromatic objective 20x/0.40  WD7.97,
Infinity LWD plan achromatic objective 40x/0.60  WD3.76
Infinity LWD plan phase contrast objective 20x/0.40  WD7.97
Focus SystemLow positioned, coaxial coarse & fine focusing system with adjustable tension control and upper limit stop, minimum reading 1μm, moving range 12mm
NosepieceQuintuple, reversed type with positive click stops
CondenserAbbe condenserN.A. 0.30 with iris diaphragm, with phase contrast slider & filter (φ34) blue,green & yellow, long work distance 72mm
StageRight hand controls, low position, mechanical stage, size 240x260mm, moving range 135x85mm.
Drop specimen holder(φ118)
Specimen holder (76×26, φ54)
Light Source6V30W Halogen lamp house
Electrical part for 6V30W halogen lmap
6V30W halogen bulb (Osram)
250V/3.15A Φ5X20 Fuse
Power supply 110-240V,50/60Hz
Fluorescence100W DC Mercury lamp house
Power supply unit for 100W DC mercury lamp
Epi-fluorescent illumination,multi-functional semi-reflector
100W Mercury bulb (Osram)
250V/5A Φ5X20 Fuse
Excitation filter B/G, CHROMA Brand
Blue Excitation Filter, BP460~490, DM500, BA520
Green Excitation Filter, BP510~550, DM570, BA590
X
Excitation filter B/G/U/V, CHROMA Brand
Blue Excitation Filter, BP460~490, DM500, BA520
Green Excitation Filter, BP510~550, DM570, BA590
–Ultraviolet Excitation Filter, BP330~385, DM400, BA420
–Violet Excitation Filter, BP400~410, DM455, BA455
X
Phase ContrastPhase contrast slider for 10x,20x,40x
Adapter0.5x, C-Mount
OtherAllen screw driver 2mm
Allen screw driver 2.5mm
Dust cover
Power cord
Optional AccessoriesItem No.
HeadSeidentopf binocular head, 45° inclined, interpupillary distance 48-75mm, diopter adjustableBIM800FLW-BH
EyepieceWF10x/22mm plan eyepiece, high eyepointBIM800FLW-10×22
WF10x/20mm, plan, high eyepoint up to 21mm, diopter adjustableBIM800FLW-10×20
WF16x/13mm, planBIM800FLW-16×13
ObjectiveInfinity plan achromatic objective 4xBIM800FLW-4xbf
Infinity LWD plan achromatic objective 60xBIM800FLW-60xLWD
Infinity LWD plan phase-contrast objective 10x (positive)BIM800FLW-10xPB
Infinity LWD plan phase-contrast objective 40x (positive)BIM800FLW-40xPB
Infinity LWD Plan Phase-Contrast Objective 10x (Negative)BIM800FLW10xNB
Infinity LWD Plan Phase-Contrast Objective 20x (Negative)BIM800FLW-20xNB
Infinity LWD Plan Phase-Contrast Objective 40x (Negative)BIM800FLW-40xNB
StageMechanical Stage, Size 350x208mm,Moving Range 50x50mmBIM800FLW-xy50
Adapter0.25x C-MountBIM800FLW-0.25XC
Digital camera adapter (need to know camera brand)BIM800FLW-DSLR-X
Bulb6V30W Halogen bulbBIM800FLW-6v30W
Specimen Holder125×85BIM800FLW-XY-125
Fluorescence Attachment100W DC Mercury lamp houseBIM800FLW-HBO
Power supply unit for 100W DC mercury lampBIM800FLW-HBO-PS
Epi-fluorescent illumination,multi-functional semi-reflectorBIM800FLW-RF
Excitation Filter B, CHROMA BrandBIM800FLW-IB
Excitation Filter G, CHROMA BrandBIM800FLW-IG
Excitation Filter U, CHROMA BrandBIM800FLW-IUV
Excitation Filter V, CHROMA BrandBIM800FLW-IV
100W Mercury bulbBIM800FLW-HBO-C
100W Mercury bulb (Osram)BIM800FLW-HBO-O
Empty Filter CubeBIM800FLW-F

Sample Images

BIM800FLW/cartilage_10xbf.jpg

10x Ph-postive lens, BF mode, 0.5x c-mount adapter

BIM800FLW/cartilage_20xbf.jpg

20x Ph-positive lens, BF mode, 0.5x c-mount adapter

BIM800FLW/cartilage_40xpo-bf-1x.jpg

40x Ph-positive lens, BF mode, 1x c-mount adapter

BIM800FLW/cartilage_60xbf-1x.jpg

60x Ph-positive lens, BF mode, 1x c-mount adapter

BIM800FLW/cartilage_10xph.jpg

10x Ph-positive lens, Ph mode, 0.5x c-mount adapter

BIM800FLW/cartilage_20xph.jpg

20x Ph-positive lens, Ph mode, 0.5x c-mount adapter

BIM800FLW/cartilage_40xph-pos-1x.jpg

40x Ph-positive lens, Ph mode, 1x c-mount adapter 

BIM800FLW/cartilage_40xph-neg-1x.jpg

40x Ph-negative lens, Ph mode, 1x c-mount adapter

Following images were collected using this microscope, BIC-C30 3MP sCMOS Camera, BIM800-0.5x 0.5x C-mount adapter and the below-mentioned objective lenses. merged images were generated using Image software.

FluoCells® Prepared Slide #2: bovine pulmonary artery endothelial cells (BPAE) cells with Mouse Anti-α-tubulin, BODIPY FL Goat Anti-Mouse IgG, Texas Red-X Phalloidin, and DAPI), (Molecular Probes)

Obj LensUV ExcitationBlue ExcitationGreen ExcitationMerged / Superimposed 2-3 Channels
20x

BIHR-20xBF

BIM800FLW/slide2-20x-bfp.jpgBIM800FLW/slide2-20x-gfp.jpgBIM800FLW/slide2-20x-rfp.jpgBIM800FLW/slide2-20x-rgb.jpg
40x

BIMJ834-40x

BIM800FLW/slide2-40x-bfp.jpgBIM800FLW/slide2-40x-gfp.jpgBIM800FLW/slide2-40x-rfp.jpgBIM800FLW/slide2-40x-rg.jpg
50x LWD

BIHR-50xBFL

BIM800FLW/slide2-50xlwd-bfp.jpgBIM800FLW/slide2-50xlwd-gfp.jpgBIM800FLW/slide2-50xlwd-rfp3.jpgBIM800FLW/slide2-50xlwd-rgb.jpg
60x

BIMJ969-60x 

BIM800FLW/slide2-60x-bfp.jpgBIM800FLW/slide2-60x-gfp.jpgBIM800FLW/slide2-60x-rfp.jpgBIM800FLW/slide2-60x-rgb.jpg

FluoCells® Prepared Slide #6 (Muntjac cells with Mouse Anti-OxPhos Complex V Inhibitor Protein, Alexa Fluor 555 Goat Anti-Mouse IgG, Alexa Fluor 488 Phalloidin, and TO-PRO-3)
(Molecular Probes)

BIM800FLW/slide6-05x-rg.jpg
5x BIHR-5xBF

BIM800FLW/slide6-40x-rg.jpg

40x BIMJ834-40x

BIM800FLW/slide6-60x-rg.jpg

60x BIMJ969-60x 

BIM800FLW/slide6-20x-rfp.jpg

20x BIHR-20xBF

BIM800FLW/slide6-20x-gfp.jpg
20x BIHR-20xBF

BIM800FLW/slide6-40x-rfp-cp.jpg

40x BIMJ834-40x  

fluorescent/BK-F2_G.JPG fluorescent/BK-F2_B.JPG

SKU: BIM-800FLW Categories: ,

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