Large Fluorescence Colony Imaging for 100mm PD and well-plate BCC-FL-100PD

Introduction

Customer Case Study: Cost-Effective Fluorescence Imaging for Petri Dishes

Customer Background: A research laboratory in US specializing in microbiology approached us with a need for a low-cost imaging setup to rapidly capture images of bacterial colonies in petri dishes (~10 cm). Their primary requirement was imaging in multiple fluorescence channels, typically using a 1x–1.25x objective.

Challenge: The customer needed a fluorescence imaging system capable of:

• Detecting fluorescence in bacterial colonies with sufficient resolution to meet scientific journal QC standards.
• Providing even illumination; experienced  both above and below illumination to ensure image clarity, i.e. inverted or upright system is fine with them.
• Capturing images with minimal stitching required, or offering an automated stitching solution.
• Supporting fluorescence channels including CFP, YFP, mCherry (red), with GFP and DAPI as additional preferences.
• Offering a cost-effective and user-friendly solution, ideally an all-in-one system, though they were open to purchasing their own camera if necessary.
• Ensuring ease of use, allowing them to focus on their research rather than complex imaging workflows.

Solution: After assessing their needs, we recommended a fluorescence imaging system that meets the following criteria:

• Fluorescence Compatibility: Equipped with filter sets optimized for CFP, YFP, mCherry, GFP, and DAPI to meet their core fluorescence requirements.
• Large Field of View: A camera system with a sufficiently wide field of view to minimize the need for image stitching, i.e. an FOV of 100mm x 100mm. Optional lens with medium size FOV at 50mm to 4mm range with live stitching technique.
• Automated Imaging (alternative): A system with motorized XY stage and autofocus capabilities to streamline the imaging process. Requires reasonable budget.
• Flexible Illumination: Adjustable illumination for optimal visibility of bacterial colonies.
• User friendly switch of filter: at a speed of click from a touch pad control.
• Cost-Effective Configuration: We provided options for an all-in-one system and a modular setup where the customer could integrate their own camera.

Outcome:

• The customer expressed interest in purchasing the second series, with the possibility of an additional unit for a colleague.
• The proposed solution met their need for fluorescence imaging at a budget-friendly price.
• The system’s automation features reduced manual effort, improving research efficiency.
• The even illumination and wide field of view ensured high-quality images suitable for publication.

This case demonstrates our commitment to delivering tailored, affordable, and high-performance imaging solutions for scientific research. If you have similar requirements, we are happy to help customize a system that fits your needs.