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IncuScope IS250 Incubator Microscope with 10x, 25x, 40x Phase Contrast and Fluorescence

$USD 3,995.00

Fits inside an incubator or a hood.
Quadruple nosepiece, 10x, 25x and 40x Ph (and FL) with optional lenses of 2x-150x, total magnifications of 100x-400x extendable to 20x-3000x (without digital zooming).
2.3MP Color USB3.0 High-Sensitive Camera, C-mount adapter and calibration slide makes the imaging bundle so professional
A trinocular head to use the scope in a classical way
USB 3.0 connections for camera and LED Control
LED Control via manual controller and software
Light off during the waiting time, i.e. digit shutter to avoid photobleaching and phototoxicity
IncuView Software for LED Control (intensity, turn on/off), video or time-lapse imaging and merging channels

Imaging Capability

Objective Lens

Camera

C-Mount Adapter

What is an IncuScope?

IncuScope is a compact inverted fluorescence microscope that fits on a shelf inside an incubator or inside a hood. It has brightfield, phase contrast and fluorescence imaging capability with optional darkfield imaging. It can be either used with camera only or with a trinocular head attached to a camera. Thus it will be a stand-alone microscope to use on the bench or keep inside an incubator for video time-lapse imaging. This system can run on a laptop or desktop using USB 3.0 or USB 2.0 cable, allows capturing images, and controlling the LEDs. It has several options of objective lenses (from 2x to 150x) and fluorescent filters (from UV to IR). The IncuScope is the most simple and affordable live cell imaging device in the market.

On the bench

Brightfield & Fluorescence

camera attachment

Inside a hood

Phase Contrast & Fluorescence

camera attachment

Inside an incubator

Fluorescence & Phase Contrast

trinocular head & camera

Applications

When you need to have a complete access of a biological microscope inside an incubator, IncuScope is a perfect choice. If you need to monitor your cells under microscope in hypoxy conditions and do not like to spend $25,000 for a miniature incubator / heating stage to add to your microscope, IncuScope IS250 will do the job you need. Some examples of IncuScope Applications are (but not limited to):

Cell Proliferation and confluency study
Cell growth, death and differentiation kinetics
Cell Counting
Cell Migration and wound healing
Cell Cycle
Stem cells development
Cancer Cells
Microfluidics study
Hypoxy Condition

Why IncuScope IS250? Why is it better than the other similar products?

The IncuScope IS250 series has several advantages over the competitors such as:

Price: it has the lowest price of the incubator microscope in the market while its image quality and performance stay superior. For instance:
- Lumascope 560 series with BF and FL imaging, single obj lens costs $7,500 USD, plus XY stage for $750 and each sample holder at $130. If you need phase contrast, add another $3,350 for single obj lens.
- EVOS, with similar package starts at $18,000
- Juli with similar package starts at$ 25,000
Image quality talks. You compare it to believe.
Flexibility in design: This incubator microscope has the most diverse flexible design. You can select any combination of camera and c-mount adapter, and any fluorescence filter design.
Unique Feature: darkfield, polarization imaging is possible. The other incubator products can not offer this.

	IncuScope 250	LumaScope 560Ph	EVOS M5000
Viewing Head	Trinocular included	Not Possible	Not available
Objective Lens	Multiple (4 or 5)	Single	Multiple (5)
Imaging Capability	BF, Ph, FL optional Darkfield & Polarizing	BF, Ph, FL	BF, FL
Monitor	Optional HD Retina Display	Not possible	Available
Camera Selection	Several options of sensor sizes and speed (or use your own)	Fixed	Fixed
C-Mount adapter	Select from 0.37x to 1.2x	Fixed	Fixed
LED Selection	Customizable from 400nm to 1500nm, over 40 filter set combination	GFP only	Configurable
Dimension (Depth x Width x Height)	311 x 200 x 428 mm 12.2 x 7.9 x 16.8 in	With XY Stage 240 x 267 x 378 mm 9.4 x 10.5 x 14.9 in	460 x 330 x 360 mm 18 x 14 x 13 in
Weight	4.5kg / 10lb	4.5kg / 10lb	16kg /35lb
Price	$7,495	Starts at $10,850	> $18,000

This video shows the video time-lapse of T Cells cultured in a 96-well plate and captured by IncuScope IS251 inside an incubator overnight (for 12 hours) using 25x Phase Contrast.Thanks Jamie McNicol at McMaster University, Hamilton, ON, Canada.
https://youtu.be/XOAXbv55iCQ

Winning Features

Small enough to fit inside an incubator or a biological hood.
Comes with Quadruple nosepiece
Phase Contrast Bundle: 10x Ph, 25x Ph and 40x Ph (suitable for FL too), 33mm WD Condenser, phase alignment tool
Optional Lenses: 2x, 2.5x, 4x, 5x, 20x, 50x, 60x, 100x, 150x BF & FL, and 20x Ph.
Single Fluorescence channel, default: GFP, with several optional customized channels (400-1500nm)
Optional darkfield and polarization imaging capability.
2.3MP Color USB3.0 High-Sensitive Camera, two C-mount adapters and calibration slide makes the imaging bundle so professional.
A trinocular head to use the scope in a classical way on the bench.
USB 3.0 connections for camera and LED Control
LED Control via manual controller and software (simultaneously)
IncuView Software for LED Control (intensity, turn on/off), time-lapse imaging and merging channels
Excellent tool for live cell imaging

Incuscope IS250 Incubator Microscope

with 10x, 25x and 40x Phase Contrast and Fluorescence Objective Lenses

1. Observation System a) Camera: several combinations of cameras and c-mount adapters can be used. A camera with 1/2″ sensor is combined with a 0.5x C-mount adapter for maximum field of view or a 1x c-mount adapter to make the total magnifications double.
b) Trincoular Head: single diopter adjustment, 30 degree inclined, comfortable and great looking. Includes the default 10x eyepieces with field number 20mm. It also has a lever to switch to camera with 100% transmission, excellent choice for fluorescence imaging without loosing any signal.
2. Mechanical Moving Stage A compact design double layer mechanical stage, with holders of 35mm petri-dish, 55mm petri-dish, glass slide, T-25 flask, etc.
3. Illumination System A high-power LED lamp is mounted inside the lamphouse serving for uniform light distribution and over 20,000 hours lifetime. Spatial positions can be adjusted easily. It has a collecting lens, field diaphragm or shutter.
4. Condenser System Plug-in Phase Contrast condenser with 30mm working distance. Optional, WD: 50mm and 72mm.
5. Phase Contrast Observation Equipped with high quality phase contrast plan objective lenses. Comes with phase alignment tool and phase annulus insert sliders.
6. Nosepiece A quadruple nosepiece is included. Optional: Quintuple.
7. Objective Lenses The system includes 10x, 25x and 40x Phase contrast lenses. Optional lenses are 2x, 4x, 5x, 10x, 20x, 40x (dry or oil), 50x (dry or oil), 60x, 100x (oil or water) and 150x (dry). High NA and High Resolution objective lenses are available.
8. Fluorescence A high-power LED is used for epi-fluorescence imaging. The default LED and filter is suitable for GFP family fluorophores. Plenty of LED and filter combinations are available, from 300nm until 1500nm. Other filters will be provided from Chorma technologies or similar one.
9. Darkfeild Imaging (Optional) The unique feature of this microscope is offering darkfield imaging capability. It comes with high NA condenser, in both dry and oil immersion formats. The 100x (oil) lens comes with Iris.

Parameter	Description	IS251	IS252
Eyepiece	Wide field WF10X/20mm	●	●
	WF16X/11mm	○	○
	Dividing 10X/20mm, 0.1mm/Div	○	○
Objective Lenses	Plan achromatic PL L 10X/0.25/WD8.8mm	●	●
	Plan achromatic PL L 25X/0.40/WD 4.8mm	●	●
	Plan achromatic PL L 40X/0.60/ WD 3.3mm	●	●
	20x Ph, and 2x, 2.5x, 4x, 5x, 20x, 60x, 100x (oil), 150x BF & FL	○	○
Eyepiece tube	Trinocular (Inclination of 30°, 100% image light for photography capable)	●	●
Focus system	Coaxial coarse/fine focus, with tensional adjustable and up stop minimum division of fine focusing: 2μm.	●	●
Nosepiece	Quadruple (Ball bearing inner locating)	●	●
Nosepiece	Quintuple (Ball bearing inner locating)	○	○
Stage	Double layer mechanical Size: 200mm x 152mm, moving range: 75mm x 30mm	●
Stage	Double layer mechanical Size: 224mm x 208mm, moving range: 112mm x 79mm		●
Sample Holders	Match to install: 68mm or 77mm x 33mm culture dish	●	○
	Match to install: 68mm culture dish	●
	Match to install: 68mm or 77mm x 29mm culture utensil		●
	Match to install: 82mm x 57mm culture utensil		●
	Well -plate 128mm x 85mmm	●	●
Filter	Blue filter	●	●
	Green filter	●	●
	Frosted filter / diffuser	●	●
	Yellow filter	○	○
Impellent style phase contrast unit	Centering telescope	●	●
	10X Impellent style phase plate	●	●
	Condenser Rack & pinion adjustable, NA=0.4,working distance: 30mm	●
	Condenser Rack & pinion adjustable, NA=0.4,working distance: 50mm	○	●
	Ultra long working distance, W.D.: 70mm	○	○
Light source	500mA white LED with brightness control.	●	●
	700mA blue LED 470nm with brightness control.	●	●
	Customizable LEDs from 400nm to 1200nm, up to 1500mA.	○	○
Fluorescence	Excitation: 482/35 BrightLine Bandpass Emission: 536/40 BrightLine Bandpass Dichroic Mirror: 506 nm BrightLine Dichroic Beam Splitter	●	●
Applicable Fluorophores	GFP (EGFP), FITC (Fluorescein), Alexa Fluor488, SuperGlo GFP, wild type GFP (non-UV excitation), red-shifted GFP (S65T), BODIPY, Cy2, Calcein, MitoTracker Green, Oregon Green.	●	●
Camera	2.3MP (1920×1200), 1/1.9″, 7.20mm x 4.5mm, 3.75 μm/pixel, USB3.0, Colourful.	●	●
Camera	*Alternative: various sensor size (1/3” -1”) and speed (up to 200 fps)	○	○
Camera C-Mount Adapter	0.37X	●	●
	0.5X	○	○
	1X	○	○
	0.5X with dividing 0.1mm/Div	○	○
PC communication	USB 3.0 port	●	●
Power	DC adapter, 12V	●	●
LED Life Time	> 20,000 hours	●	●
Operating System	Microsoft Windows 7, 8, 10	●	●
Operating Condition	0°C- 42°C, 5%-99% RH non-condensing	●	●
Size / Dimension	195 x 311 x 428 mm / 7.7 x 12.2 x 16.8 in (Width x Depth x Height) 200 (with stage) x 445 (with viewing head) x428mm (H) / 7.9 x 12.2 x 16.8 in	●
Size / Dimension	195 x 350 x 468 mm (Width x Depth x Height) / 7.7 x 13.8 x 18.4 in 267 (with stage) x 478 (with viewing head) x 468mm (H) / 10.5 x 18.8 x 18.4 in		●
Warranty	1 Year	●	●
Warranty	Extended Warranty for 2-5 years	○	○

Note: ● Standard Outfit, ○ Optional, * Contact Bioimager for consultation

Cameras:

Order Code	Sensor & Size (mm)	Pixel (μm)	G Sensitivity Dark Signal	FPS/Resolution	Binning	Exposure
BIC-E3S-2.0	2.0MP IMX385(C) 1/2″(7.20×4.05)	3.75 x3.75	2350mv with 1/30s 0.15mv with 1/30s	125 @1920×1080	1×1	0.1ms ~15s
BIC-E3C-2.3	2.3MP IMX185(C) 1/1.9″ (7.20×4.50)	3.75×3.75	1120mv with 1/30s 0.5mv with 1/30s	38@1920×1200 66@960×600	1×1, 2×2	0.244ms~2s
BIC-E3C-2.3G	2.3MP IMX249 (C,GS ) 1/1.2“ (11.25×7.03)	5.86×5.86	1016mv with 1/30s 0.15mv with 1/30s	30@1920×1200	1×1	0.244ms~2s
BIC-E3S-3.1G	3.1MP IMX265 (C, GS) 1/1.8″(7.07×5.30)	3.45×3.45	1146mv with 1/30s 0.15mv with 1/30s	53 @2048×1536 85 @1024×768	1×1	0.1ms ~15s
BIC-E3S-5G	5.0MP IMX264(C, GS) 2/3” (8.45×7.07)	3.45×3.45	1146mv with 1/30s 0.15mv with 1/30s	35 @2448×2048 50 @1224×1024	1×1	0.1ms ~15s
BIC-E3S-6.3	6.3MP* IMX178(C) 1/1.8″(7.37×4.92)	2.4×2.4	425mv with 1/30s 0.15mv with 1/30s	30 @3072×2048 38 @1536×1024	1×1,2×2	0.1ms ~15s
BIC-E3S-9.0G	9M IMX305 (C, GS) 1″(14.13×7.45)	3.45 x3.45	1146mv with 1/30s 0.15mv with 1/30s	34@4096×2160 60@2048×1080	1×1, 2×2,	0.1ms ~15s
BIC-E3S-20.0	20MP IMX183(C) 1″ (13.06×8.76)	2.4 x 2.4	462mv with 1/30s 0.21mv with 1/30s	15@5440×3648 50 @2736×1824 60@1824×1216	1×1, 2×2, 3×3	0.1ms~15s

* Suitable only for brightfield and phase contrast-imaging.

**: C: Color, GS: Global Shutter, M: Monochrome

When we have two LEDs then software captures 5 images at every time point:

1) phase contrast (or brightfield), Monochrome

2) phase contrast (or brightfield), pseudo-color (like white or slightly grey if you pick)

3) Fluorescence image, monochrome

4) fluorescence image, pseudo-color (like green fro GFP)

5) merged color images (image #2 +image #4)

Video 1: Review of IS250 IncuScope Microscope Optomechanics and functions

Video 2: Sample Time-Lapse Viedeo of T-Cells

Sample Images:

Example 1: These images were taken with 10x Phase contrast. Image #1 and #2 are identical and just one was presented.

10x Phase Contrast

10x Fluorescence, Monochrome

10x Fluorescence, labeled green (pseudo colour)

Merged / Superimposed image of phase contrast and fluorescence

Imaging Modes:

Brightfield
Phase contrast
Fluorescence
Darkfield
Polarization

Cameras:

Order Code	Sensor & Size (mm)	Pixel (μm)	G Sensitivity Dark Signal	FPS/Resolution	Binning	Exposure
BIC-E3S-2.0	2.0MP IMX385(C) 1/2″(7.20×4.05)	3.75 x3.75	2350mv with 1/30s 0.15mv with 1/30s	125 @1920×1080	1×1	0.1ms ~15s
BIC-E3C-2.3	2.3MP IMX185(C) 1/1.9″ (7.20×4.50)	3.75×3.75	1120mv with 1/30s 0.5mv with 1/30s	38@1920×1200 66@960×600	1×1, 2×2	0.244ms~2s
BIC-E3C-2.3G	2.3MP IMX249 (C,GS ) 1/1.2“ (11.25×7.03)	5.86×5.86	1016mv with 1/30s 0.15mv with 1/30s	30@1920×1200	1×1	0.244ms~2s
BIC-E3S-3.1G	3.1MP IMX265 (C, GS) 1/1.8″(7.07×5.30)	3.45×3.45	1146mv with 1/30s 0.15mv with 1/30s	53 @2048×1536 85 @1024×768	1×1	0.1ms ~15s
BIC-E3S-5G	5.0MP IMX264(C, GS) 2/3” (8.45×7.07)	3.45×3.45	1146mv with 1/30s 0.15mv with 1/30s	35 @2448×2048 50 @1224×1024	1×1	0.1ms ~15s
BIC-E3S-6.3	6.3MP* IMX178(C) 1/1.8″(7.37×4.92)	2.4×2.4	425mv with 1/30s 0.15mv with 1/30s	30 @3072×2048 38 @1536×1024	1×1,2×2	0.1ms ~15s
BIC-E3S-8.3	8.3MP IMX485(C) 1/1.2″(7.68×4.32)	2.9×2.9	2188mv 0.15mv	45 @3840×2160 70 @1920×1080	1×1 2×2	0.02ms -15s
BIC-E3S-9.0G	9M IMX305 (C, GS) 1″(14.13×7.45)	3.45 x3.45	1146mv with 1/30s 0.15mv with 1/30s	34@4096×2160 60@2048×1080	1×1, 2×2,	0.1ms ~15s
BIC-E3S-20.0	20MP IMX183(C) 1″ (13.06×8.76)	2.4 x 2.4	462mv with 1/30s 0.21mv with 1/30s	15@5440×3648 50 @2736×1824 60@1824×1216	1×1, 2×2, 3×3	0.1ms~15s
BRC-6012**	6MP 1/2.8″			30fps@ 3264 × 1836 on SD card to LCD monitor, 60fps @1920 × 1080 (HDMI cable) 30fps@ 3264 × 1836 (USB Cable) with software to PC

* Suitable only for brightfield and phase contrast-imaging.

**HD Camera with 11.6″ Retina Display. Not supported by IncuView yet. Further Info.

System Diagram:

IncuView Software Interface

Features:

Time-lapse mode
Controls each LED separately
Turn ON/OFF option for the LEDs, i.e. digital shutter
Live view and image capture
Label image color
Merge images
Color and monochrome images
Manual and automatic exposure time and gain
Captures BF and FL images separately
Zoom in / Zoom out with a touch of mouse wheel
Full screen of the live image, resizable tabs

For Image Analysis Software, please check Image Pro page.

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IncuScope IS250 Incubator Microscope with 10x, 25x, 40x Phase Contrast and Fluorescence

What is an IncuScope?

Applications

Why IncuScope IS250? Why is it better than the other similar products?

Winning Features

Incuscope IS250 Incubator Microscope

with 10x, 25x and 40x Phase Contrast and Fluorescence Objective Lenses

1. Observation System

2. Mechanical Moving Stage

3. Illumination System

4. Condenser System

5. Phase Contrast Observation

6. Nosepiece

7. Objective Lenses

8. Fluorescence

9. Darkfeild Imaging (Optional)

Cameras:

Video 1: Review of IS250 IncuScope Microscope Optomechanics and functions

Video 2: Sample Time-Lapse Viedeo of T-Cells

Sample Images:

Imaging Modes:

Cameras:

System Diagram:

Reviews

Browse

Want to chat?

Social

IncuScope IS250 Incubator Microscope with 10x, 25x, 40x Phase Contrast and Fluorescence

What is an IncuScope?

Applications

Why IncuScope IS250? Why is it better than the other similar products?

Winning Features

Incuscope IS250 Incubator Microscope

with 10x, 25x and 40x Phase Contrast and Fluorescence Objective Lenses

1. Observation System

2. Mechanical Moving Stage

3. Illumination System

4. Condenser System

5. Phase Contrast Observation

6. Nosepiece

7. Objective Lenses

8. Fluorescence

9. Darkfeild Imaging (Optional)

Cameras:

Video 1: Review of IS250 IncuScope Microscope Optomechanics and functions

Video 2: Sample Time-Lapse Viedeo of T-Cells

Sample Images:

Imaging Modes:

Cameras:

System Diagram:

Reviews

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