IncuScope IS500 Incubator Microscope: Small, Smart & Simple
What is an IncuScope?
The IncuScope is a low price, small, compact, USB-based, portable digital inverted brightfield and epi-fluorescence microscope. The microscope fits on a shelf inside an incubator or inside a hood. It has a built-in digital camera and light source. This runs on a laptop or desktop using just one USB cable and captures fluorescence and/or brightfield images. Available with a variety of objective lenses from 1.25x to 100x. The software allows doing time-lapse study and controlling the light to turn on/off to avoid photo-bleaching and photo-toxicity. Thus, this saves paying for heating stage or miniature incubator, mechanical shutter, digital camera and fluorescence attachment which are important components for live cell imaging application. Its price is the lowest in the live cell imaging field. A side-by-side comparison with similar products such as Lumascope, Juli, EVOS, this has better images. When it comes to the price, this is the most economic choice. Last but not least, you can check your cell culture inside an incubator via Internet or smart-phone if you connect the IncuScope computer to Internet / WiFi and use our remote-control software.
Contact Us for Free Demo, a Quote , and Custom Design with 6 colors or OEM.
Why IncuScope?
There is no greatness where there is not simplicity Leo Tolstoy, quoted in The week.
- Simple: the most simple digital microscope in the world, requires no setup, almost no training, instant installation, familiar PC environment.
- Affordable: The lowest price video time-lapse microscope in the world.
- Your cells are always happy since they never leave incubator.
- Using our remote-control software/app you can see inside your incubator via Internet or iPhone, Android, Blackberry
Highlighted Applications:
- Long-term live cell imaging in single and time lapse modes
- Cell doubling and movement measurements
- Stem cell differentiation movies
- More sophisticated uses are also easily achieved with the IncuScope: cell counting or hemocytometry and growth monitoring. Using the IncuScope, it is possible to monitor cells remotely through the web from within a tissue culture incubator, overnight, away from the lab.
Unlimited Scientific and Routine Applications
- Microfluidics field, lab-on-chip, MEMS
- Viral infections, HIV/AIDS study and all level III safety labs
- Embryology, In Vitro Fertilization (IVF) studies
- Bacteriology
- Stem Cell Colony formation study (long term live cell imaging)
- Transfections, knock-out studies such as siRNA tagged
- Drug discovery research and clinical labs
- Field microscopy, such as marine biology
- Proliferation assay
- Kinetic cell migration assay
- Kinetic angiogenesis
- Cell invasion
- Cytotoxicity
- Cell culture quality control
- Apoptosis
Colorful brigtfield images (available with iS500BF model only)
- Yields a magnification on familiar laptop displays of approximately 20× with a field of view of 1 mm square.
- Standard filter sets
- Brightfield capability included. Phase Contrast is optional.
- Time-lapse study with turn on/off light (without using a shutter!)
- USB 3.0 interface, enabling easy integration with larger systems and remote monitoring
- Small enough to fit on a desk, lab bench, in an incubator or tissue culture hood (approximately 190 x 125 x 115 mm or 7.5 x 5 x 4.5 in).
- #1 Performance/Price in the Market; it saves paying to heating stage, shutter and camera, e.g. compare it with Zeiss
Price: The best price in the imaging market:
- LumaScope LS500 ( > $8,000)
- Juli (> $15,000)
- EVOS (> $27,000)
- IncuCyte (> $77,000)
Compact & Turn-Key Imaging Solutions:IncuScope
What is IncuScope?
IncuScope is a compact inverted fluorescence microscope that fits on a shelf inside an incubator or inside a hood. It has a built-in camera and LED light source. This system runs on a laptop/desktop using just one USB cable, allows capturing fluorescence and bright field images, and has several options of objective lenses and fluorescent filters. The IncuScope is the most simple and affordable live cell imaging device in the market.
Features
- Fits inside a hood or an incubator (saves heating stage, operates in natural environment of incubator and sterile condition)
- USB-based camera & LED illumination
- Avoids photo-toxicity & photo-bleaching (digital shutter)
- The best performance/price live cell imaging device in the market
- Extremely simple and user-friendly software & hardware
- Remote control (browser based, iPhone or Android Apps to see inside an incubator!)
|
IncuScope-500 |
| Optics |
Infinity-corrected objective with an RMS thread type |
| Image Resolution |
1920×1200 pixels = 2.3 MP; 1/1.9″(7.20×4.5)
CMOS Sensor (3.75×3.75 microns/pixels) |
| Image Formats |
JPEG, TIF, BMP, GIF, PNG. |
| Optics |
Chroma or Omega Optical Filters |
| Image Size |
100 kB-4MB |
| Objectives |
1.25x, 2x, 4x, 5x, 10x, 20x, 40x, 50x (oil), 60x (dry), 100x (water, or air/oil) (optional) |
| Fluorescence
(Default) |
Excitation: 482/35 BrightLine Bandpass
Emission: 536/40 BrightLine Bandpass
Dichroic Mirror: 506 nm BrightLine Dichroic Beam Splitter |
| Fluorophores |
GFP (EGFP), FITC (Fluorescein), Alexa Fluor488, SuperGlo GFP, wild type GFP (non-UV excitation), red-shifted GFP (S65T), BODIPY, Cy2, Calcein, MitoTracker Green, Oregon Green. |
| PC communication |
USB 3.0 port |
| Power |
DC adapter |
| LED Life Time |
> 20,000 hours |
| Operating System |
Microsoft Windows 7, 8, 10 |
| Operating Condition |
0°C- 42°C, 5%-99% RH non-condensing |
| Warranty |
1 year |
Field of Views
| Field of View |
1.25x: 19x19mm
2x: 9.31x7mm
4x: 7.8×5.85mm
5x: 6.8×5.11mm
10x: 3.4×2.55mm
20x: 1.52×1.14mm
40x: 7.8×5.85mm |
Colorful brigtfield images (available with iS500BF model only)
|
BIM5-4x 4X Plan Achromat, Cartilage, 16MP Camera |
4x Plan Semi Apo, Cartilage, 16MP Camera |
|
BUM5-10x 10x Plan Semi Apo, Cartilage, 16MP Camera |
![""](//www.bioimager.com/media/wysiwyg/IncuScope/incuscope-20xmj0.7-cartil.jpg") 20x Plan Apo, Cartilage, 16MP Camera |
![""](//www.bioimager.com/media/wysiwyg/IncuScope/incuscope-40x-bim500-cartilage.jpg")
BIM5-40x 40x Plan Obj lens, Cartilage, 16MP Camera |
![""](//www.bioimager.com/media/wysiwyg/IncuScope/incuscope-50xhv-cartilage.jpg")
50x Plan Semi Apo Obj lens, Cartilage, 16MP Camera |
Following images were collected using 3 LEDs of UV, Blue and Green excitations, 3MP sCMOS camera BIC-C30, and the below-mentioned objective lenses. merged images were generated using IncuView software.
FluoCells® Prepared Slide #2: bovine pulmonary artery endothelial cells (BPAE) cells with Mouse Anti-α-tubulin, BODIPY FL Goat Anti-Mouse IgG, Texas Red-X Phalloidin, and DAPI), (Molecular Probes)
BIHR-20xBF PlanFluor EPI20XBF Plan Semi Apochromat 20X/NA0.45/WD3 Lens:
BIMJ969-60x: 60x /NA0.80/WD0.23 Plan Objective Lens:
FluoCells® Prepared Slide #6 (Muntjac cells with Mouse Anti-OxPhos Complex V Inhibitor Protein, Alexa Fluor 555 Goat Anti-Mouse IgG, Alexa Fluor 488 Phalloidin, and TO-PRO-3) (Molecular Probes)
BIMJ969-60x: 60x /NA0.80/WD0.23 Plan Objective Lens:
Microscope Models:
| Model |
Description |
| iS510 |
Brightfield and Epifluor Imaging (Per specification table)
includes iS500-OEM plus focus, enclosure
no obj lens, no XY stage |
| iS500BF |
iS500 with Brightfield imaging Only |
| iS510-OEM |
Brightfield and Epifluor Imaging
includes just the epi-fluorescnce module (filter cube, filters), BF and FL LEDs, camera, PCB and software |
| iS510T |
iS500 with Tablet PC or Retina Display |
| iS520 |
iS500 with BF and 2 Fl channels |
| iS530 |
iS500 with BF and 3 FL channels |
IncuScope Accessories
- Customized any fluorescence filters from UV to Infrared
- Various Camera Selections, i.e. different resolution (1.4MP to 16MP), speed (1-90 fps) and chip size (1/3”-1”).
- Objective lenses: 1.25x, 2x, 4x, 5x, 0x, 20x, 40x, 50x (oil), 60x (dry), 100x (water, or air/oil)
1. LED Selection
You select the LEDs, we will select the proper excitation emission and barrier filters.
| LED |
Nominal
Wavelength |
Minimum LED
Power Output |
Applications/ Fluorophores |
| Deep UV |
265 nm |
10 mW |
|
| Deep UV |
280 nm |
25 mW |
|
| Deep UV |
300 nm |
40 mW |
|
| Deep UV |
340 nm |
53 mW |
|
| UV |
365 nm |
190 mW |
|
| UV |
365 nm |
1150 mW |
|
| UV |
375 nm |
387 mW |
Dapi/Hoecst |
| UV |
385 nm |
270 mW |
|
| UV |
385 nm |
1650 mW |
|
| UV |
395 nm |
400 mW |
|
| UV |
405 nm |
870 mW |
|
| UV |
405 nm |
1500 mW |
|
| Violet |
420 nm |
750 mW |
|
| Royal Blue |
450 nm |
1850 mW |
|
| Royal Blue |
455 nm |
900 mW |
|
| Blue |
470 nm |
650 mW |
GFP |
| Blue |
490 nm |
255 mW |
|
| Cyan |
505 nm |
400 mW |
|
| Green |
530 nm |
350 mW |
|
| Lime |
565 nm |
880 mW |
|
| Amber |
590 nm |
160 mW |
RFP/ Texas Red |
| Amber |
595 nm |
445 mW |
|
| Orange |
617 nm |
600 mW |
|
| Red |
625 nm |
700 mW |
|
| Deep Red |
660 nm |
940 mW |
|
| Far Red |
730 nm |
515 mW |
|
| IR |
780 nm |
200 mW |
|
| IR |
780 nm |
800 mW |
|
| IR |
810 nm |
325 mW |
|
| IR |
850 nm |
900 mW |
|
| IR |
850 nm |
1400 mW |
|
| IR |
880 nm |
300 mW |
|
| IR |
940 nm |
800 mW |
|
| IR |
970 nm |
35 mW |
|
| IR |
1050 nm |
50 mW |
|
| IR |
1200 nm |
30 mW |
|
| IR |
1300 nm |
25 mW |
|
| IR |
1450 nm |
31 mW |
|
| IR |
1550 nm |
31 mW |
|
| Broadband |
470 – 850 nmf |
70 mW |
|
| Warm White |
3000 Kg |
570 mW |
Brightfield |
| Warm White |
3000 Kg |
2000 mW |
|
| Cold White |
6500 Kg |
800 mW |
|
| Cold White |
6500 Kg |
2350 mW |
|
2. Cameras
| Order Code |
Sensor & Size (mm) |
Pixel (μm) |
G Sensitivity
Dark Signal |
FPS/Resolution |
Binning |
Exposure |
| BIC-T3C-12C |
12MP / IMX226(C)
1/1.7″ (7.40×5.55) |
1.85×1.85 |
280mv with 1/30s
0.1mv with 1/30s |
Q3/coming soon |
1×1,2×2 |
0.1ms~2000ms |
| BIC-T3C-6C |
6.3MP / IMX178(C)
1/1.8″ (7.37×4.92) |
2.4×2.4 |
425mv with 1/30s
0.15mv with 1/30s |
15@3072×2048
26@1536×1024 |
1×1,2×2 |
0.244ms~2000ms |
| BIC-T3C-3C2 |
3.1MP/IMX123(C)
1/2.8″(6.46) |
2.5×2.5 |
600mv with 1/30s
0.15mv with 1/30s |
25@2048×1536
30@1024×768 |
1×1,2×2 |
0.244ms~2000ms |
| BIC-T3C-2C1
(Default) |
2.3M / IMX185(C)
1/1.9″(7.20×4.5) |
3.75×3.75 |
1120mv with 1/30s
0.5mv with 1/30s |
30@1920×1200 |
1×1,2×2 |
0.244ms~2000ms |
| BRC-1600 |
16MP / MN3412 (Color)
1/2.33″ |
1.335 x 1.335 |
1200mV |
4fps @4608×3456
16 @ 2304 x1728 |
1×1,2×2 |
1ms-999ms |
We also offer CCD and sCMOS cameras, either USB 3.0 or USB2.0. Please contact us for further assistance.
3. Obj lenses
Types:
a) plan achromat
b) plan semi-apo
c) Plan apo
Magnifications: 1.25x, 2x, 4x, 5x, 10x, 20x, 40x, 50x, 60x and 100x (oil, dry or water)
4. Accessories
| Mechanical stage can be installed from both right and left sides easily, even from the front. |
The mechanical XY stage grabs a micro-well plate directly without having any holder) |
| all customized shape of sample holders |
T-25 Flask holder T-75 Flask holder are also available. |
| dimensions for the Terasaki holder. 35-mm petri-dish holders needs this adapter. |
|
incuView Software Interface
Features:
Time-lapse mode
Controls each LED separtely
Turn ON/OFF option for teh LEDs, i.e. digital shutter
Live view and image capture
label image color
Merge images
Color and monochrome images
Manual and automatic exposure time and gain
Captures BF and FL images separately
Zoom in / Zoom out with a touch of mouse wheel
Full screen of the live image, resizable tabs
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1.25x Obj Lens
2x Obj Lens
4x
5x
10x
20x
50x obj lens with 2.3MP camera
![""](http://www.bioimager.com/media/wysiwyg/IncuScope/incuscope-20xmj0.7-cartil.jpg")
20x Plan Apo, Cartilage, 16MP Camera![""](http://www.bioimager.com/media/wysiwyg/IncuScope/incuscope-40x-bim500-cartilage.jpg")
![""](http://www.bioimager.com/media/wysiwyg/IncuScope/incuscope-50xhv-cartilage.jpg")
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