BIM410RPC/FL Hoffman/Relief Phase Contrast & Fluorescence Inverted Biological Microscope
– Inverted Biological Microscope
– Brightfield, and optional Hoffman Modulation Contrast/Relief Contrast and/or Fluorescence
– 4x, 10x, 20x, 40x objective lenses
– 10x/22 eyepieces, 40x-400x total magnifications
– Optional C-Mount Adapters: 0.35x, 0.5x, 0.65x or 1xSKU: n/a
BDS-200L Large 3D Imaging System
- High Performance digital microscope
- High Resolution (0.1um in 30mm travel) 3D Images of large samples
- Optical magnifications from 0.45 to 20x and total magnification of 25x-1000x
- Great tool for 3D imaging of large specimens (0.1-10mm) such as minerals, jewelries, industrial inspection of PCB, small animals such as zebrafish, chicken, fruits, seeds, etc.
- Output image format for 3D printing
- Epi-Fluorescence imaging of small animals (optional)
A reliable microscope is an essence for effective diagnosis and professional measurements. The microscopist performs the inspection of materials, prepare an analysis report then a supervisor makes a proper decision. There are so many details on the objects that cannot be seen by a regular microscope. Suitable microscopy parts, optomechanical components, camera, and software are required to achieve such a goal.
BIOIMAGER’s metallurgical microscopes offer high magnification with reflected and transmitted light. Metallograph microscopes allow the user to view opaque items at high magnification. Specialty uses for metallurgical microscopes include use as measuring instruments for measuring thin films, electroplating coatings, grain size, surface inclusions and defects. Our metallurgical microscopes are available in binocular and trinocular models with both upright, inverted and boom stand microscope systems.
Metallurgical microscopes are available with brightfield only or brightfield and darkfield. There is a great explanation of imaging modes here and metallurgical microscopy basics and application here. The high magnification of metallurgical microscopes is perfect for viewing items that won’t allow light to pass through them (such as metals, coated films and solid objects where defects must be detected.) All metallurgical microscopes have the option to add a microscopy camera and software for capturing images and making measurements.
1. Metallurgical Microscope Types
Metallurgical microscopes are divided mainly to 4 categories:
1) Upright Microscopes with reflected light only, in which the light comes from top lamp-house and is used for non-transparent samples
2) Upright Metallurgical Microscopes with reflected and transmitted lights, in which light can come from top and bottom light sources and can be used to examine the transparent and non-transparent samples.
3) Inverted metallurgical Microscopes which includes a lamp house for reflected illumination. Bioimager offers economic external light source to be able to use for transparent samples
4) Boom Stand Metallurgical Microscopes
|Upright Metallurgical Microscope with Reflected light only||Upright Metallurgical Microscope with Reflected & Transmitted||Inverted Metallurgical Microscopes||Boom Stand Metallurgical Microscopes|
Please select the products from the left side category list or search bar.
2. Imaging Capabilities
a) BF or DF? By default all metallurgical microscopes come with brightfield (BF) imaging. If you need to have darkfield (DF) imaging, you do need to decide during the purchase time as upgrading such a feature will not only be costly but also will result in loosing the money you spent on BF module and also the obj lenses. Switching between BF and DF is always super easy, just move a lever.
b) DIC Nomarsky or not? DIC Nomarsky imaging needs a polarizer and analyzer. Make sure your microscope does / will come with polarizer, analyzer, slot for DIC insert and also which objective lenses are supported for DIC imaging.
Having DIC Nomarsky is always costly. If your microscope allows having a DIC imaging but your budget does not permit, make sure you can upgrade that by simply purchasing the extra parts without discarding your current obj lenses or setup.
3. The 3D Profiler
one of the most interesting and useful feature of the metallurgical microscopes is having a 3D profiler which allows measurement of the height and/or thickness of a sample. This is a great tool for inspection of the materials or scaffold are made in three-dimensional, such as 3D tissue or dental cements. BIOIMAGER has recently added an output format of .stp to the constructed 3D microscopy images then you can easily print out what you observe under microscope.
|3D Profile with automated Z focus at 10 nm resolution||3D Profiler with tilting stand|
4. Illumination Guide for Transmitted & Reflected Light
|Specimen Type||Imaging Technique|
|Transparent Specimens – Bacteria, spermatozoa, cells in glass containers, protozoa, mites, fibers, etc.||Phase Contrast|
|Light Scattering Objects – Diatoms, fibers, hair, fresh water microorganisms, radiolarians, etc||Darkfield Illumination|
|Light Reflecting Specimens –|
Colloidal suspensions, powders and minerals, liquids
|Amplitude Specimens –|
Stained Tissue, naturally colored specimens, hair & fibers, insects and marine algae
|Fluorescent Specimens –|
Cells in tissue culture, fluorochrome-stained sections, smears and spreads
|Birefringent Specimens –|
Mineral thin sections, liquid crystals, melted and re-crystallized chemicals, hairs & fibers, bones & feathers
|Specimen Type||Imaging Technique|
|Specular (Reflecting) Surfaces –|
Thin film, mirrors, polished metallurgical samples, integrated circuits
|Diffuse (Non-Reflecting) Surfaces – Thick and thin films, rocks & minerals, hair, fiber, bone and insects||Brightfield Illumination|
|Amplitude Surface Features –|
Dyed fibers, diffuse metallic specimens, composite materials, polymers
|Birefringent Specimens – Mineral thin sections, hair & fibers, bones & feathers, single crystals, oriented films||Polarized Illumination|
|Fluorescent Specimens –|
Mounted cells, fluorochrome-stained sections, smears and spreads
5. Analysis and Measurements
|Field / Sample||Image||Attributes, Measurement Features, Analysis|
|Ceramics, composites, Plastic|
|Particles & Porous materials|
|Glass, films, coatings|
|Textile / Fibers|
Analysis Example: Fiber Separation Analysis
|The application is designed to separate and measure crossing fibers on images. The fibers on the images can be straight or bent. It can be used to measure distribution of thin inclusions or asbestos fibers.|
Researchers have been looking for an easier way to measure the lengths and widths of overlapping fibers. Most researchers end up manually measuring fibers – a process that is not only time consuming, but can introduce inconsistencies. Now, with the Image-Pro Premier Fiber Separation App, it is possible to measure and classify the length and thickness of overlapping fibers with one click.
This App is designed for applications where the entire fiber is visible in the image.
6. Sample Images / Applications
|Sample: Duplex steel, damaged rotor blade etching: Beraha III, Camera: ProgRes® C5, Courtesy of: Cloeren Technology/Germany||Sample: PC board, Camera: ProgRes® C14, Courtesy of: ProgRes® Application Lab||Sample: Blower grinding tool, Camera: ProgRes® C14plus, Courtesy of: ProgRes® Application Lab|
|Sample: stone on stereo microscopes Camera: ProgRes® C7, Courtesy of: ProgRes® Application Lab||Sample: compound from graphite and powder, Camera: ProgRes® C3, Courtesy of: Hoffmann & Co. Elektrokohle AG, Austria||Sample: fluid channel fo a press button Camera: ProgRes® 3012, Courtesy of: HSG-IMIT Microtechnology|
|Sample: detail of a SQUID (superconducting quantum interference device), Camera: ProgRes® C3, Courtesy of: MPQ of Radio Astronomy, Bonn, Germany||Sample: rolled metal sheet, Camera: ProgRes® C14, Courtesy of: MPI for Iron Research GmbH||Sample: Wafer im DIC Camera: ProgRes® C14plus Courtesy of: Promicron|
|Sample: Cast Iron (spherulitic graphite iron), Camera: ProgRes® C5, Courtesy of: Cloearen Technology, Germany||Sample: new formation of cristobalite in glass, Camera: ProgRes® C14plus, Courtesy of: Institute of Ceramic, Glass and Building Materials of the Mining Academy||Sample: TWIP Steel, Camera: ProgRes® C14, Courtesy of: MPI for Iron Research GmbH|
7. Video Tutorial: How To Use a Compound Light Microscope
See Polarized and DIC Imaging Video tutorials at their pages.